The mouse Anti-5-Methylcytosine monoclonal antibody (clone 10G4) has been developed to facilitate differentiation between methylated and non-methylated cytosines in DNA. Specificity of this clone is to 5-methylcytosines in single-stranded DNA with no detectable crossreactivity to non-methylated cytosines. The antibody has proven to be a valuable tool in the characterization of DNA methylation and has been successfully used for immunopreciptiation-based assays such as Methylated DNA Immunoprecipitation (MeDIP).
|Flow Cytometry||N/D *||N/A *|
|Immunohistology||N/D *||N/A *|
|Immunoprecipitation (IP)||Yes||2-4 µg per IP|
|Western Blotting||N/D *||N/A *|
* N/A = Data not available. N/D = Not Determined
|Storage||Store at 0 to 4° C with prolonged storage at -80° C. Avoid repeated freeze/thawing.|
|Specificity||5-Methylcytosine in single stranded DNA from any source including human, rat, mouse, plants, etc. and 5-Methylcytidine|
|Approximate Protein Concentration||1 mg/ml|
|Buffer Solution||Antibody is provided in PBS (Phosphate Buffered Saline, pH 7.4) with 0.05% Sodium azide as a preservative.|
|Product Type||Monoclonal Antibody|
Anti-5mC antibodies were used in a southwestern dot blot assay to detect 5-methylcytosine in genomic DNA from E. coli expressing DNA methyltransferases (MTases) from various bacterial strains. Dot blots were then visualized and the hybridization signals were quantified to compare the DNA methylation activity of the different MTases.