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E. coli Non-methylated Genomic DNA

Product Size Catalog # Price Qty
E. coli Non-methylated Genomic DNA 5 µg / 20 µl D5016

Concentration 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Applications Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.
Genotype ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2
Source Escherichia coli strain ER2925* (Dam¯ and Dcm¯) [*Used with permission from New England Biolabs.]

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