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Human WGA Methylated & Non-methylated DNA Set

Purified, methylated & non-methylated human WGA (Whole Genome Amplified) DNA is ideal for use as controls for many methylation detection applications.
Included primers are designed to amplify non-methylated, methylated, and mixed methylation copies of the Ras association (RalGDS/AF-6) domain family member 1 (RASSF1) gene following bisulfite conversion

Product Size Catalog # Price Qty
Human WGA Methylated & Non-methylated DNA Set 1 Set D5013
Human WGA Non-Methylated DNA 5 μg/20 μl D5013-1
Human WGA Methylated DNA 5 μg/20 μl D5013-2

About Human WGA Methylated & Non-methylated DNA Set

The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation™, EZ DNA Methylation-Gold™, EZ DNA Methylation-Direct™, and EZ DNA Methylation-Lightning™ kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA.

The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.

Concentration 250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)
Source Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.
Storage -20 °C

Figure 1. An assay for complete methylation by M.SssI methytransferase. Digestion of non-methylated and methylated WGA DNA with restriction enzymes MspI and HpaII. MspI digests both non-methylated and methylated DNA. HpaII is sensitive to CpG methylation.

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