About Human WGA Methylated & Non-methylated DNA Set
The Human WGA Methylated & Non-methylated DNA Set consists of two control DNAs (non-methylated and methylated) along with a set of specifically designed primers that can be used in conjunction with the EZ DNA Methylation™, EZ DNA Methylation-Gold™, EZ DNA Methylation-Direct™, and EZ DNA Methylation-Lightning™ kits from Zymo Research to assess the efficiency of bisulfite-mediated conversion of DNA.
The Human WGA Methylated & Non-methylated DNA Set is generated using phi29 DNA polymerase based whole genome amplification techniques from HCT116 DKO cell line derived genomic DNA (Human HCT116 DKO Non-methylated DNA). The Human WGA Methylated DNA is Human WGA Non-methylated DNA that has been enzymatically methylated at all double-stranded CG dinucleotides using M.SssI methyltransferase and can be used as a positive control for DNA methylation analysis.
|Concentration||250 ng/µl in buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0)|
|Source||Whole genome amplified DNA from HCT116 DKO cells. Human WGA Methylated DNA is enzymatically methylated by M.SssI methyltransferase.|
Figure 1. An assay for complete methylation by M.SssI methytransferase. Digestion of non-methylated and methylated WGA DNA with restriction enzymes MspI and HpaII. MspI digests both non-methylated and methylated DNA. HpaII is sensitive to CpG methylation.