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dNTP Mix

Ready to use dNTP Mix (dATP, dTTP, dGTP, dCTP) of ultra high purity; >99% trisphosphate by HPLC
Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities

Product Size Catalog # Price Qty
dNTP Mix 10 mM D1000
dATP 100 mM D1005
dTTP 100 mM D1010
dGTP 100 mM D1015
dCTP 100 mM D1020

About dNTP Mix

dNTP Mix and dATP, dTTP, dGTP, dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.

Reagent Volume Final Concentration
2X Reaction Buffer 25 µl 1X
dNTP Mix 0.5 µl 0.25 mM each dNTP
Forward Primer (10 µM) Variable 0.3 to 1 µM
Reverse Primer (10 µM) Variable 0.3 to 1 µM
Template DNA Variable < 200 ng/50 µl
ZymoTaq™ DNA Polymerase (5 U/µl) 0.4 µl 2 U/50 µl
ddH20 to 50 µl -
Total Volume 50 µl  
Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.

Concentration dNTP Mix: 5 µmol/500 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and dCTP).
dATP, dTTP, dGTP, dCTP: 25µmol/250 µl as a [100 mM] solution.
Storage dNTP Mix and dATP, dTTP, dGTP, dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.

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