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dNTP Mix

Ready to use dNTP Mix (dATP, dTTP, dGTP, dCTP) of ultra high purity; >99% trisphosphate by HPLC
Readily incorporated into PCR amplicons with ZymoTaq™ or other DNA polymerases
Free of endo-, exodeoxyribonuclease, ribonuclease, phosphatase and nicking activities

Product Size Catalog # Price Qty
dNTP Mix 10 mM D1000
$26.00
dATP 100 mM D1005
$46.00
dTTP 100 mM D1010
$46.00
dGTP 100 mM D1015
$46.00
dCTP 100 mM D1020
$46.00

About dNTP Mix

dNTP Mix and dATP, dTTP, dGTP, dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.

Reagent Volume Final Concentration
2X Reaction Buffer 25 µl 1X
dNTP Mix 0.5 µl 0.25 mM each dNTP
Forward Primer (10 µM) Variable 0.3 to 1 µM
Reverse Primer (10 µM) Variable 0.3 to 1 µM
Template DNA Variable < 200 ng/50 µl
ZymoTaq™ DNA Polymerase (5 U/µl) 0.4 µl 2 U/50 µl
ddH20 to 50 µl -
Total Volume 50 µl  
 
Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.


Concentration dNTP Mix: 5 µmol/500 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and dCTP).
dATP, dTTP, dGTP, dCTP: 25µmol/250 µl as a [100 mM] solution.
Storage dNTP Mix and dATP, dTTP, dGTP, dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.

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