About dNTP Mix
dNTP Mix and dATP, dTTP, dGTP, dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
|2X Reaction Buffer||25 µl||1X|
|dNTP Mix||0.5 µl||0.25 mM each dNTP|
|Forward Primer (10 µM)||Variable||0.3 to 1 µM|
|Reverse Primer (10 µM)||Variable||0.3 to 1 µM|
|Template DNA||Variable||< 200 ng/50 µl|
|ZymoTaq™ DNA Polymerase (5 U/µl)||0.4 µl||2 U/50 µl|
|ddH20||to 50 µl||-|
|Total Volume||50 µl|
|Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations.|
|Concentration||dNTP Mix: 5 µmol/500 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and dCTP).
dATP, dTTP, dGTP, dCTP: 25µmol/250 µl as a [100 mM] solution.
|Storage||dNTP Mix and dATP, dTTP, dGTP, dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.|
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