About dNTP Mix
dNTP Mix and dATP, dTTP, dGTP, dCTP from Zymo Research are of ultra high purity and can be used to generate DNA by PCR using ZymoTaq™ or other DNA polymerases.
| Reagent | Volume | Final Concentration |
| 2X Reaction Buffer | 25 µl | 1X |
| dNTP Mix | 0.5 µl | 0.25 mM each dNTP |
| Forward Primer (10 µM) | Variable | 0.3 to 1 µM |
| Reverse Primer (10 µM) | Variable | 0.3 to 1 µM |
| Template DNA | Variable | < 200 ng/50 µl |
| ZymoTaq™ DNA Polymerase (5 U/µl) | 0.4 µl | 2 U/50 µl |
| ddH20 | to 50 µl | - |
| Total Volume | 50 µl | |
| Note: The final concentration of MgCl2 in the reaction (above) is 1.75 mM. If required, scale reaction reagent volumes accordingly to optimize the MgCl2, primer, and/or template concentrations. | ||
Specifications
| Concentration | dNTP Mix: 5 µmol/500 µl as a [10 mM] solution. Contains [2.5 mM] of each dNTP (dATP, dTTP, dGTP and dCTP). "¢ dATP, dTTP, dGTP, dCTP: 25 μmol/250 μl as a [100 mM] solution. |
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| Storage | dNTP Mix and dATP, dTTP, dGTP, dCTP are guaranteed for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C. |
