Rapid, robust, and simple purification of high quality, inhibitor-free DNA from any sample including feces, soil, water, biofilms, swabs, saliva, body fluids, etc.
ZymoBIOMICS™ innovative lysis system enables efficient and unbiased lysis of microbes including Gram-positive and negative bacteria, fungus, protozoans, algae, and viruses.
Unbiased extraction of ultra-pure DNA makes the ZymoBIOMICS™ DNA Mini Kit ideal for 16S rRNA gene sequencing, shotgun metagenomic sequencing, arrays, PCR and other sensitive applications.

Product Size Catalog # Price Qty
ZymoBIOMICS™ DNA Miniprep Kit 5 Preps D4300T
$40.00
ZymoBIOMICS™ DNA Miniprep Kit 50 Preps D4300
$290.00
ZymoBIOMICS™ DNA Miniprep Kit (Lysis Matrix Not Included) 50 Preps D4304
$202.00

About ZymoBIOMICS™ DNA Miniprep Kit

The ZymoBIOMICS™ DNA Miniprep Kit is designed for purifying DNA from a wide array of sample inputs (e.g. feces, soil, water, and biofilms), that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS™ innovative lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Unbiased mechanical lysis of tough microbes is achieved by bead beating with the innovative ZymoBIOMICS™ ultra-high density BashingBeads™ and validated using the ZymoBIOMICS™ Microbial Community Standard, as shown in Figure 3. In addition, the ZymoBIOMICS™ DNA Miniprep kit is equipped with Zymo’s Proprietary OneStep™ PCR Inhibitor removal technology enabling PCR from the most PCR prohibitive environmental samples rich in humic and fulvic acids, tannins, melanin, and other polyphenolic compounds. Coupling state-of-the-art lysis technology with Zymo-Spin™ purification technology results in superior yields of ultra-pure DNA ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing.


DNA Recovery Up to 25 µg total DNA can be eluted into 100 µl (50 µl minimum) ZymoBIOMICS™ DNase/RNase Free Water
Equipment Microcentrifuge, vortex/Disruptor Genie®, high speed cell disrupter (recommended)
Sample Sources Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, and 50 – 100 mg (wet weight) of fungal/bacterial cells, biofilms and water
DNA Purity High quality, inhibitor-free DNA is eluted with ZymoBIOMICS™ DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation sequencing
Bead Beating System ZymoBIOMICS™ innovative lysis system enables complete homogenization/disruption of the microbial cells walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis it is recommend that the ZymoBIOMICS™ Microbial Community Standard is used for calibration of each bead beating device
DNA Integrity Generally, post bead beating, genomic DNA has an average size of 15-20 kb depending on the initial quality of the sample making it amenable to Next-Gen sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield™
Bioburden A single preparation is guaranteed to contain less than 3 bacterial genomic copies per 1 µl of eluate as determined by quantitative amplification of the 16S rRNA gene when eluted using 100 µl water.

Figure 1. The ZymoBIOMICS™ DNA Mini Kit provides unbiased representation of the organisms extracted from the ZymoBIOMICS™ Microbial Community Standard. DNA was extracted from ZymoBIOMICS™ Microbial Community Standard using four different DNA extraction methods (ZymoBIOMICS™ DNA Mini Kit, Human Microbiome Project Protocol, Supplier M, and Supplier Q) and analyzed using 16S rRNA gene sequencing. 16S rRNA genes were amplified with primers targeting v3-4 region and the amplicons were sequenced on Illumina® MiSeq™ (2x250bp). Overlapping paired-end reads were assembled into complete amplicon sequences. The composition profile was determined based on sequence counts after mapping amplicon sequences to the known 16S rRNA genes of the eight different bacterial species.

Figure 2. The ZymoBIOMICS™ DNA Mini Kit reliably isolates DNA from even the toughest to lyse gram positive organisms, enabling unbiased analyses of microbial community compositions. There is a significant increase in yield and Gram-positive abundance when DNA was isolated using the ZymoBIOMICS™ DNA Mini Kit. Correlated with the results in Figure 3A it can be concluded that unbiased DNA isolation was achieved. DNA was extracted from 200 μl of human feces suspended in PBS (10 % m/v) using four different DNA extraction methods (ZymoBIOMICS™ DNA Mini Kit, Human Microbiome Project Protocol, Supplier M, and Supplier Q) and analyzed using 16S rRNA gene sequencing. 16S rRNA genes were amplified with primers targeting v3-4 region and the amplicons were sequenced on Illumina® MiSeq™ (2x250bp). Overlapping paired-end reads were assembled into complete amplicon sequences. Amplicon sequences were profiled with Qiime using Greengenes 16S rRNA gene database (gg_13_8).

Figure 3. The ZymoBIOMICS™ DNA Mini Kit provides inhibitor-free DNA even when challenged with extremely inhibitor rich samples. Real-time PCR was used to evaluate eluates recovered using the ZymoBIOMICS™ DNA Mini Kit, or Suppliers M, P, and Q. Reaction volumes consisted of either 10% or 35% of the eluate from each kit to detect the presence of PCR inhibitors. Each reaction contained 25 ng of Brettanomyces DNA. Delayed and/or no amplification indicates PCR inhibition from inefficient inhibitor removal.

Figure 4. The ZymoBIOMICS™ DNA Mini Kit provides superior yields when compared to Suppliers M, P, and Q. 80 mg of feces was processed using each kit according to the manufactures’ recommended protocol. DNA was eluted using 100 μl. 6 μl of each sample was analyzed in a 1.0% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate. L is a 1Kb ladder.

Figure 5. The ZymoBIOMICS™ DNA Mini Kit can be used to isolate high quality DNA from a variety of soil types which yields robust products following PCR. Panel A: Physical characteristics of sampled soils (1-5) (Reg. 1). Panel B: Microbial DNA was isolated from soil samples (1-5) using the ZymoBIOMICS™ DNA Mini Kit. Approximately 10% of the eluted DNA was then separated in a 0.8% (w/v) agarose/ethidium bromide gel. Panels C and D show the results of PCR of microbial DNA isolated from the samples with primers specific for prokaryotic 16S rRNA (C) or eukaryotic rRNA (D). In the figures, the 1 kb size marker (NEB) is as indicated and the arrows show the prokaryotic 16S rRNA and eukaryotic rRNA PCR products.

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