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| • | Quick (2 minute) desalting and recovery of ultra-pure DNA from enzymatic reactions (e.g., PCR and endonuclease digestions), cell-free lysates, etc. |
| • | Column design allows DNA to be eluted at high concentrations into minimal volumes of solvent. |
| • | Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc. |
| Product | Catalog No. | Size | Price | Store |
|---|---|---|---|---|
| DNA Clean & Concentrator™-25 (uncapped columns) |
D4005 | 50 Preps. | $ | |
| D4006 | 200 Preps. | $ | ||
| DNA Clean & Concentrator™-25 (capped columns) |
D4033 | 50 Preps. | $ | |
| D4034 | 200 Preps. | $ | ||
| Components Available | ||||
| • | Format: Spin Column |
| • | Sample Sources: DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. |
| • | DNA Size Limits: 50 bp to 23 kb |
| • | DNA Recovery: Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. |
| • | DNA Purity: High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • | Equipment Needed: Microcentrifuge |
The DNA Clean & Concentrator™-25 (DCC™-25) is designed for rapid desalting and purification of up to 25 µg DNA from enzymatic reactions (e.g., PCR), endonuclease digestions, or cell-free lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ column. The product features Fast-Spin column technology to yield high-quality, purified DNA in just minutes, and it is compatible with cDNA and ssDNA. Eluted DNA is suitable for sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
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The DNA Clean & Concentrator™ yields high quality DNA for efficient transcription reactions. Lanes: M: 1 kb Marker; A: DNA template purified using the DNA Clean & Concentrator™; B: a 7 kb RNA transcript generated in vitro from A.
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