DNA Clean & Concentrator™- 500

 
• Simple, rapid recovery of ultra-pure DNA from large-scale sample sources.
• Unique column construction allows sample washing to be performed using a centrifuge or vacuum source.
• Column design allows DNA to be eluted at high concentrations into minimal volumes.
• Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, arrays, etc.
 
Product Catalog No. Size Price Store
DNA Clean & Concentrator™-500 D4031 10 Preps. $
  D4032 20 Preps. $
 
Components Available
 
• Format: Spin Column.
• Sample Sources: DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates.
• DNA Size Limits: From 50 bp to 23 kb.
• DNA Recovery: Typically, ≤ 500 µg total DNA can be eluted with ≥ 1 ml water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%.
• DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.
• Product Detergent Tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS.
• Equipment: Microcentrifuge and centrifuge, vacuum source, or syringe.
 

The DNA Clean & Concentrator™-500 (DCC™-500) is our highest capacity DNA Clean & Concentrator™ product. It is designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples such as large-scale restriction endonuclease digestions and crude DNA preparations. Eluted DNA is well suited for use in PCR, DNA sequencing, DNA transfection, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. The entire DNA purification/concentration procedure typically takes less than 20 minutes.

 

 
 

Ultra-pure DNA from the DNA Clean & Concentrator™ can be easily ligated for efficient cloning. Lanes: A: 3.5 kb blunt-ended vector; B: 7.4 kb blunt-ended DNA insert; C: Blunt-end ligation of A+B mixture.