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| • | Make your own highly efficient chemically competent cells: 108-109 transformants/μg of plasmid DNA for most common lab strains. |
| • | No heat shock or related procedures: simply add DNA and spread onto a plate - Mix & Go! |
| Product | Catalog No. | Price | Store | |
|---|---|---|---|---|
| Z-Competent E. coli Transformation Kit™ (E. coli growth medium ZymoBroth™ is supplied.) |
T3001 | $ | ||
| Z-Competent E. coli Transformation Buffer Set™ (E. coli growth medium ZymoBroth™ is NOT supplied.) |
T3002 | $ | ||
| Components Available | ||||
The Z-Competent™ E. coli Transformation Kit and Z-Competent™ E. coli Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Z-Competent™ method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go! bacterial transformation can be performed by adding DNA to Z-Competent™ cells and spreading onto a plate. Transformation efficiencies are typically on the order of 108-109 transformants/μg plasmid DNA with most E. coli strains.
Uniquely formulated reagents make it easy to generate Z-Competent™ cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Z-Competent™ E. coli Transformation Kit includes all buffers and ZymoBroth™ medium to generate 20 ml of Z-Competent™ cells. The Z-Competent™ E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Z-Competent™ cells, and the medium (broth) is supplied by the user.
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