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| • | Quick (15 minute) RNA isolation from a variety of sources using Fast-Spin column technology. |
| • | RNAlater™ compatible. |
| • | Omits the use of organic denaturants and proteases. |
| Product | Catalog No. | Size | Price | Store |
|---|---|---|---|---|
| ZR RNA MicroPrep™ | R1060 | 50 Preps. | $ | |
| R1061 | 200 Preps. | $ | ||
| Free Sample |
R1060S | 5 Preps. | Free | |
| Components Available | ||||
| • | Format: Spin Column |
| • | Sample Sources: Cells from culture or small amounts of solid tissue. |
| • | Sample Size: 101 - 105 cells in suspension or solid form. |
| • | RNA Purity: High quality RNA (A260/A280>1.8, A260/A230>1.8) is recovered. |
| • | RNA Recovery: RNA can be eluted into small volumes, ≥ 6 µl allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~5 µg. |
| • | Storage: ≤-70ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| • | Processing Time: 15 min (does not account for in-column DNase I treatment (~20 min)) |
| • | Equipment Needed: Microcentrifuge |
The ZR RNA MicroPrep™ provides a quick method for high quality total RNA isolation from cells, needle biopsies, and tissue. The product isolates both large and small RNA species without the use of reducing agents or phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered with a simple adjustment of the RNA isolation protocol. Eluted RNA is suitable for use in RT-PCR and other RNA-based procedures.
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Recovery of small RNA. Total RNA isolated using the ZR RNA MicroPrep™ was resolved in an agarose gel (2-4) and small RNAs from the same sample were also resolved in a native polyacrylamide gel (6-7). Input = 105 yeast cells spiked with 1 µg ZR small-RNA™ Ladder (Zymo Research)
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PCR amplification of β-actin transcript post-RT (353 bp fragment shown): Total RNA from human epithelial cells (HCT 116) was isolated using the ZR RNA MicroPrep™.
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