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| • | Quick (15 minute) recovery of large-sized DNA (e.g., genomic, plasmid [BAC/PAC], viral, phage, etc.) from agarose gels. |
| • | Unique column design for low volume (≥ 10 μl) elution of ultra-pure, high-yield DNA. |
| • | Eluted DNA is well suited for use in endonuclease digestion, sequencing, labeling, PCR, etc. |
| Product | Catalog No. | Size | Price | |
|---|---|---|---|---|
| Zymoclean™ Large Fragment DNA Recovery Kit | D4045 | 25 Preps. | $ | |
| D4046 | 100 Preps. | $ | ||
| Zymoclean™ Gel DNA Recovery Kit (uncapped columns) |
D4001 | 50 Preps. | $ | |
| D4002 | 200 Preps. | $ | ||
| Zymoclean™ Gel DNA Recovery Kit (capped columns) |
D4007 | 50 Preps. | $ | |
| D4008 | 200 Preps. | $ | ||
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ZR-96 Zymoclean™ Gel DNA Recovery Kit |
D4021 | 2x96 Preps. | $ | |
| D4022 | 4x96 Preps. | $ | ||
| Components Available | ||||
| • | Format: Spin Column |
| • | Sample Sources: DNA excised from TAE and TBE-buffered agarose gels. |
| • | DNA Size Limits: 50 bp to ~200 kb |
| • | DNA Recovery: Typically, up to 10 μg total DNA per column can be eluted into ≥ 10 μl water. For DNA 50 bp to 23 kb the recovery is 70-90%. For DNA ≥ 23 kb, the recovery is 50-70%. |
| • | DNA Purity: High-quality, purified DNA is eluted with water making it especially well suited for sequencing and ligation reactions. |
| • | Detergent Tolerance: ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS |
| • | Equipment Needed: Microcentrifuge |
The Zymoclean™ Large Fragment DNA Recovery Kit provides a streamlined method for the rapid purification and concentration of high-quality large-sized DNA from agarose gels. Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containing a DNA sample, let dissolve, and then transfer to the supplied Zymo-Spin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.
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Recovery of large DNA fragments. The Zymoclean™ Large Fragment DNA Recovery Kit was used to recover λ DNA digested with HindIII and separated by agarose gel electrophoresis. Lane C: λ-HindIII digest; lanes 1 & 3: recovered 23 kb λ-HindIII fragments; lanes 2 & 4: recovered 9 kb λ-HindIII fragments. Lane λ: intact λ phage DNA; lanes 5, 6: intact λ ~48 kb bands.
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