DESCRIPTION The Pinpoint Slide DNA Isolation System™ is an innovative product designed by Zymo Research to easily isolate genomic DNA in any targeted microscopic tissue area on a slide. The system combines our powerful Pinpoint tissue sampling technology with a one-step DNA extraction method and makes targeted tissue genomic DNA isolation easy and simple. The kit efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis. Unlike the UV-fractionation method, the Pinpoint system does not destroy the surroundings of target cells. There is no need for expensive specialized equipment or computer software. Method Principle: As outlined in Figure 2, simply apply the Pinpoint Solution to a selected microscopic tissue area. The solution will air-dry into a thin film while the underlying cells will become embedded within. The area is lifted and transferred into a tube. After digestion with the one-step Extraction Buffer (containing proteinase K) the DNA is ready for PCR analysis.

Figure 1. PCR amplified DNA fragments from different samples of paraffin-embedded tissue. Lane M: 100 bp marker; Lane 1 and 2: ß-Globin; Lane 3 and 4: An uncharacterized gene on chromosome 3.