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ZR
Fecal DNA Kit™ (Free
Sample Kit Available)
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Rapid method for the isolation of inhibitor-free, PCR-quality
DNA from fecal samples in as little as 15 minutes including
humans, birds, rats, mice, cattle, etc. |
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State-of-the-art,
ultra-high density BashingBeads™
are fracture resistant and chemically inert. |
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Can
be used with any bead mill, disrupter, or vortex that
can accommodate standard 2.0 ml tubes. |
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Omits
the use of organic denaturants as well as proteinases.
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SPECIFICATIONS
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Formats: Bead Beating, Spin Column. |
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Sample
Sources: Bacterial, protist, as well as host DNA can
be effectively isolated from a
≤
150 mg sample of mammalian feces. |
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DNA
Purity: High quality, inhibitor-free DNA is eluted
with DNA Elution Buffer suitable for the amplification of bacterial,
protist, and/or mammalian templates. Absorbance 260 nm/280
nm > 1.8. |
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DNA
Size Limits: > 1 kb. |
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DNA
Recovery: Typically, up to 25 µg total DNA is
eluted into 100 µl (25 µl minimum) DNase-Free Water
per sample. For DNA 75 bp to 10 kb, the recovery is 70-90%.
For DNA 11 kb to 23 kb the recovery is 50-70%. |
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Equipment:
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
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DESCRIPTION
The ZR Fecal DNA Kit™ is
designed for the simple, rapid isolation of inhibitor-free,
PCR-quality DNA from a variety of sample sources including
humans, birds, rats, mice, cattle, etc. The procedure is
easy and can be completed in as little as 15 minutes: fecal
samples (≤150
mg each) are added directly to a ZR BashingBead™
Lysis Tube and rapidly and efficiently lysed without
the use of organic denaturants or proteinases. The DNA is
then isolated and purified using our Fast-Spin column technology
and is ideal for downstream molecular-based applications
including PCR, arrays, etc. The DNA is also well suited
for methylation profiling purposes using any of our EZ
DNA Methylation Gold Kit™. A schematic of the
ZR Fecal Kit™ procedure is shown
to the right.
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Comparison
of DNA yields from rat feces using the ZR Fecal
DNA Kit™ and kits from competitors M
and Q. Equivalent amounts of feces were processed using
each kit and then equal volumes of eluted DNA were analyzed
in a 0.8% (w/v) agarose/ethidium bromide gel. Samples
were processed in triplicate. |
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PCR
of DNAs from rat and human fecal samples isolated
with the ZR Fecal DNA Kit™.
Panels A and B
show the results of PCR with DNA isolated
from rat and human fecal samples, respectively,
using primers specific for prokaryotic 16S
rRNA. Panel C shows the results
of PCR of DNA isolated from human feces with
and without the addition of E. coli
containing pUC19 plasmid DNA (indicated at
the top of the image) using primers specific
for the pUC19 sequence. In each case, amplicons
were analyzed in a 1.5% (w/v) agarose/ethidium
bromide gel using a UV imager. Numbers above
each lane of the gel images are the volumetric
equivalent (in µl) of eluted DNA (100
µl) used for PCR. Arrows mark the relative
migration of amplicons in the gels, and M
is a 100 bp DNA ladder (NEB). |
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
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ZR Fecal DNA Kit™
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Simple,
rapid isolation of PCR-quality DNA from fecal samples
in as little as 10 minutes. |
|
D6010 |
50
preps. |
$182.00 |
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*Free
kit offer valid online only and while supplies last.
Limit 1 per U.S. customer only. |
| Components
Available Separately |
| ZR
Bashing Bead Lysis Tubes |
S6002-50 |
50
tubes |
$92.00 |
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| Lysis
Solution |
D6001-3-40 |
40
ml |
$25.00 |
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| Fecal
DNA Binding Buffer |
D6010-1-100 |
100
ml |
$102.00 |
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| DNA
Pre-Wash Buffer |
D3004-5-15 |
15
ml |
$8.00 |
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| Fecal
DNA Wash Buffer |
D6010-2-50 |
50
ml |
$15.00 |
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DNA Elution Buffer |
D3004-4-10 |
10
ml |
$12.00 |
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| Zymo-Spin
IV Filters
(Orange Tops) |
C1007-50 |
50
filters |
$50.00 |
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| Zymo-Spin
IV-HRC Filter
(Green Tops) |
C1010-50 |
50
filters |
$92.00 |
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| Zymo-Spin
IIC Columns |
C1011-50 |
50
columns |
$45.00 |
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| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
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| C1001-500 |
500
tubes |
$30.00 |
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| C1001-1000 |
1,000
tubes |
$50.00 |
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| Lysis
Instruments Available Separately |
| Disruptor
Genie™ (120V w/ 2 ml tube holder assembly) |
S6001-120 |
1
unit |
$425.00 |
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| Disruptor
Genie™ (240V w/ 2 ml tube holder assembly) |
S6001-240 |
1
unit |
$425.00 |
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TurboMix
Attachment, 2.0 ml
Permanently mounts to most existing Vortex-Genie
2 and Vortex-Genie 2T mixers converting them to a Disruptor
Genie™. |
S6004-2 |
1
unit |
$172.00 |
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| FastPrep®-24
Instrument |
S6005 |
1
unit |
$7,625.00 |
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HiPrep™
Attachment
(Accomodates
48 x 2 ml tubes) |
S6005-1 |
1
unit |
$1,200.00 |
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CryoPrep™
Attachment
(Accomodates
24 x 2 ml tubes) |
S6005-2 |
1
unit |
$1,550.00 |
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TeenPrep™
Attachment
(Accomodates
12x 15 ml tubes) |
S6005-3 |
1
unit |
$1,200.00 |
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BigPrep™
Attachment
(Accomodates
2 x 50 ml tubes)
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S6005-4 |
1
unit |
$1,200.00 |
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