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ZR
Fungal/Bacterial DNA Kit™ (Free
Sample Kit Available)
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Easy isolation of high quality DNA from fungus or bacteria
in as little as 10 minutes. |
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Simple,
efficient isolation of DNA from all types of tough-to-lyse
fungi (e.g., yeast) and bacteria. |
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State-of-the-art,
ultra-high density BashingBeads™ are fracture resistant
and chemically inert. |
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Omits
the use of organic denaturants as well as proteinases.
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SPECIFICATIONS
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Formats: Bead Beating, Spin Column. |
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Sample
Sources: Fungi and yeast. |
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DNA
Purity: High quality DNA is eluted with DNA Elution
Buffer or water which is especially well suited for PCR. |
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DNA
Size Limits: > 1 kb. |
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DNA
Recovery: Typically, up to 25 µg total DNA is
eluted into 100 µl (25 µl minimum) water per sample.
For DNA 75 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb
to 23 kb the recovery is 50-70%. |
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Equipment:
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
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DESCRIPTION
The ZR Fungal/Bacterial DNA Kit™
is designed for the simple, rapid isolation of DNA from
tough-to-lyse fungi, including A. fumigatus, C.
albicans, N. crassa, S. cerevisiae,
S. pombe, as well as from mycelium and Gram (+)
and (-) bacteria. The procedure is easy and can be completed
in as little as 15 minutes: fungal and/or bacterial samples
are added directly to a ZR BashingBead™ Lysis
Tube and rapidly and efficiently lysed without
using organic denaturants or proteinases. The DNA is isolated
and purified using our Fast-Spin column technology and is
ideal for downstream molecular-based applications including
PCR, array, etc. A schematic of the ZR Fungal/Bacterial
DNA Kit™ procedure is shown to the right. |
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
|
ZR Fungal/Bacterial DNA Kit™
|
Simple,
rapid isolation of PCR-quality DNA from tough-to-lyse
fungi and bacteria. |
|
D6005 |
50
preps. |
$132.00 |
|
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| |
*Free
kit offer valid online only and while supplies last.
Limit 1 per U.S. customer only. |
| Components
Available Separately |
| Fungal/Bacterial
DNA Binding Buffer |
S6005-1-100 |
100
ml |
$102.00 |
|
| Lysis
Solution |
D6001-3-40 |
40
ml |
$25.00 |
|
| DNA
Pre-Wash Buffer |
D3004-5-15 |
15
ml |
$8.00 |
|
| Fungal/Bacterial
DNA Wash Buffer |
D6005-2-50 |
50
ml |
$15.00 |
|
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DNA Elution Buffer |
D3004-4-10 |
10
ml |
$12.00 |
|
| ZR
Bashing Bead Lysis Tubes |
S6002-50 |
50
tubes |
$92.00 |
|
| Zymo-Spin
IV Filters
(Orange Tops) |
C1007-50 |
50
filters |
$50.00 |
|
| Zymo-Spin
IIC Columns |
C1011-50 |
50
tubes |
$45.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
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| Lysis
Instruments Available Separately |
| Disruptor
Genie™ (120V w/ 2 ml tube holder assembly) |
S6001-120 |
1
unit |
$425.00 |
|
| Disruptor
Genie™ (240V w/ 2 ml tube holder assembly) |
S6001-240 |
1
unit |
$425.00 |
|
TurboMix
Attachment, 2.0 ml
Permanently mounts to most existing Vortex-Genie
2 and Vortex-Genie 2T mixers converting them to a Disruptor
Genie™. |
S6004-2 |
1
unit |
$172.00 |
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| FastPrep®-24
Instrument |
S6005 |
1
unit |
$7,625.00 |
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HiPrep™
Attachment
(Accomodates
48 x 2 ml tubes) |
S6005-1 |
1
unit |
$1,200.00 |
|
CryoPrep™
Attachment
(Accomodates
24 x 2 ml tubes) |
S6005-2 |
1
unit |
$1,550.00 |
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TeenPrep™
Attachment
(Accomodates
12x 15 ml tubes) |
S6005-3 |
1
unit |
$1,200.00 |
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BigPrep™
Attachment
(Accomodates
2 x 50 ml tubes)
|
S6005-4 |
1
unit |
$1,200.00 |
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