Zymo Research - ZR Genomic DNA I Kit

ZR Genomic DNA I Kit™

HIGHLIGHTS

•	Pure total DNA from either solid or liquid sources in 15 minutes.
•	Recover total DNA in pure water.
•	Easy scale up or down procedure for different sizes of samples, such as 1 µl to 10 ml of blood.
•	Safe with common anti-coagulants (EDTA, Heparin, Citrate).
•	High quality DNA for PCR, Southern Blots, restriction digests etc.
•	Extract DNA from fresh blood immediately or samples can be stabilized in the Genomic Lysis Buffer for delayed processing during blood storage and transport.
•	No organic extraction, precipitation, or proteinase K digestion.

SPECIFICATIONS

•	DNA Purity: High-purity DNA is recovered in water which is especially good for subsequent experiments.
•	Recovery Volume: 35 µl for the standard procedure, but can be adjusted according sample size.
•	DNA Size: > 40 kb for genomic DNA, mitochondria DNA is also recovered.
•	Stability: Quality is guaranteed for 1 year from the purchase date.

DESCRIPTION

The ZR Genomic DNA Kit is based on a simple one buffer procedure for rapid genomic DNA isolation. The kit is optimized to produce high quality DNA without RNA contamination. The ZR Genomic DNA Kit is designed for whole fresh blood, whole stored blood, frozen blood, buffy coat, bone marrow tissue cultured cells, solid tissue, buccal cells, cell culture, and liquid samples. The uniquely formulated Genomic Lysis Buffer directly lyses the cells and releases the DNA. The procedure then relies upon the rapid adsorption of the released DNA onto the surface of the Zymobeads in the buffer at the same time. The DNA/Zymobead complex is separated by brief centrifugation and wash steps are performed to remove contaminants. DNA is eluted in water. The yield of DNA is quantitative, reproducible and the DNA is of good purity for direct molecular biology applications such as PCR, restriction enzyme digest, cloning, DNA sequencing, Southern Blot analysis, etc.

The ZR Genomic DNA Kit is based on a simple one buffer procedure for rapid genomic DNA isolation. The kit is optimized to produce high quality DNA without RNA contamination. The ZR Genomic DNA Kit is designed for whole fresh blood, whole stored blood, frozen blood, buffy coat, bone marrow tissue cultured cells, solid tissue, buccal cells, cell culture, and liquid samples. The uniquely formulated Genomic Lysis Buffer directly lyses the cells and releases the DNA. The procedure then relies upon the rapid adsorption of the released DNA onto the surface of the Zymobeads in the buffer at the same time. The DNA/Zymobead complex is separated by brief centrifugation and wash steps are performed to remove contaminants. DNA is eluted in water. The yield of DNA is quantitative, reproducible and the DNA is of good purity for direct molecular biology applications such as PCR, restriction enzyme digest, cloning, DNA sequencing, Southern Blot analysis, etc.

ORDERING INFORMATION:

Products	Protocol	Cat No.	Size	Price
ZR Genomic DNA Kit™ (Silica bead format) Reagents provided are for 100 preps.	PDF	D3004	1 kit	$72.00
ZR Genomic DNA Kit™ (Silica bead format) Reagents provided are for 400 preps.	PDF	D3005	1 kit	$270.00

Components Available Separately
Genomic Lysis Buffer	D3004-1-50	50 ml	$30.00
Genomic Lysis Buffer	D3004-1-100	100 ml	$52.00
DNA Pre-Wash Buffer	D3004-5-15	15 ml	$8.00
	D3004-5-30	30 ml	$17.80
	D3004-5-50	50 ml	$22.00
g-DNA Wash Buffer	D3004-2-50	50 ml	$15.00
g-DNA Wash Buffer	D3004-2-100	100 ml	$26.00
Zymobeads	D3004-3-1	1 ml	$20.00
Zymobeads	D3004-3-4	4 ml	$75.00
DNA Elution Buffer	D3004-4-4	4 ml	$6.00
DNA Elution Buffer	D3004-4-16	16 ml	$16.00

RELATED PRODUCTS:

Products	Cat No.	Size	Price
ZR Genomic DNA II Kit™ (Uncapped spin column format) Reagents provided are for 50 preps.	D3006	1 kit	$72.00
ZR Genomic DNA II Kit™ (Uncapped spin column format) Reagents provided are for 200 preps.	D3007	1 kit	$250.00
ZR Genomic DNA II Kit™ (Capped spin column format) Reagents provided are for 50 preps.	D3024	1 kit	$81.00
ZR Genomic DNA II Kit™ (Capped spin column format) Reagents provided are for 200 preps.	D3025	1 kit	$263.00