Zymo Research - ZR Plant/Seed DNA Kit™

ZR Plant/Seed DNA Kit™ (Free Sample Kit Available)

HIGHLIGHTS

•	Simple method for the isolation of inhibitor-free, PCR-quality DNA from a variety of plant and seed samples in as little as 15 minutes.
•	The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, endonuclease digestion, etc.
•	State-of-the-art, ultra-high density BashingBeads™ are fracture resistant and chemically inert.
•	Can be used with any bead mill, pulverizer, or vortex that can accommodate standard 2.0 ml tubes.

SPECIFICATIONS

•	Formats: Bead Beating, Spin Column.
•	Sample Sources: Plant DNA that is free of polyphenols, tannins, and polysaccharides can be effectively isolated from a ≤ 150 mg plant sample.
•	DNA Yield: Typically 20-80 ng DNA/mg plant material.
•	DNA Purity: High quality, inhibitor-free DNA is eluted with DNA Elution Buffer that is suitable for PCR amplification. Absorbance 260 _{260 nm/280 nm} > 1.8
•	DNA Size Limits: > 1 kb.
•	DNA Recovery: Typically, up to 25 µg total DNA is eluted into 50-100 µl (25 µl minimum) DNA Elution Buffer per sample. For DNA 75 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.
•	Equipment: Microcentrifuge, vortex, cell disruptor/pulverizer (optional).

DESCRIPTION

The ZR Plant/Seed DNA Kit™ is designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in as little as 15 minutes: plant samples (≤ 150 mg each) are added directly to a ZR BashingBead™ Lysis Tube and rapidly and efficiently lysed without the use of organic denaturants or proteinases. Polysaccharides and polyphenols/tannins are removed from the DNA using our Fast-Spin column and Zymo-Spin™ IV-HRC Column technologies, respectively. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc. A schematic of the ZR Plant/Seed DNA Kit™ procedure is shown to the right.

Figure 1. Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA Kit™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research Corp.). 1 Arabidopsis thaliana 2 juniper 3 milkweed leaf 4 milkweed leaflet 5 milkweed preflowering bud 6,7 corn kernel 8,9 sunflower seed.

Figure 1. Comparison of DNA yields from various plant and seed samples using the ZR Plant/Seed DNA Kit™. Equivalent amounts of plant materials were processed with equal volumes of eluted DNA analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 1 kb DNA size marker (Zymo Research).

Figure 2. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo-Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)

Figure 2. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo- Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)

Figure 3. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from Arabidopsis thaliana leaf samples demonstrates the effectiveness of the Zymo-Spin™ IV-HRC Column at removing PCR inhibitors from the DNA. The arrow shows the relative migration of a ~700 bp amplicon from Chromosome 1 in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)

Figure 3. PCR of diluted DNA (0 to 0.001) isolated with the ZR Plant/Seed DNA Kit™ from corn kernels. The arrow shows the relative migration of a ~450 bp amplicon from mitochondrial DNA in a 0.8% (w/v) agarose/ethidium bromide gel. M is a 100 bp DNA size marker (Zymo Research Corp.)

ORDERING INFORMATION:

Products	Description	Protocol	Cat No.	Size	Price
ZR Plant/Seed DNA Kit™	Simple, rapid isolation of humic-free, PCR-quality DNA from plant and seed samples.	PDF	D6020	50 preps.	$182.00

*Free kit offer valid online only and while supplies last. Limit 1 per U.S. customer only.

Components Available Separately
ZR Bashing Bead Lysis Tubes	S6002-50	50 tubes	$92.00
Lysis Solution	D6001-3-40	40 ml	$25.00
Plant/Seed DNA Binding Buffer	D6020-1-100	100 ml	$102.00
DNA Pre-Wash Buffer	D3004-5-15	15 ml	$8.00
Plant/Seed DNA Wash Buffer	D6020-2-50	50 ml	$15.00
DNA Elution Buffer	D3004-4-10	10 ml	$12.00
Zymo-Spin IV Filters (Orange Tops)	C1007-50	50 filters	$50.00
Zymo-Spin IV-HRC Filter (Green Tops)	C1010-50	50 filters	$92.00
Zymo-Spin IIC Columns	C1011-50	50 columns	$45.00
Collection Tubes	C1001-50	50 tubes	$5.00
	C1001-500	500 tubes	$30.00
	C1001-1000	1,000 tubes	$50.00

Lysis Instruments Available Separately
Disruptor Genie™ (120V w/ 2 ml tube holder assembly)	S6001-120	1 unit	$425.00
Disruptor Genie™ (240V w/ 2 ml tube holder assembly)	S6001-240	1 unit	$425.00
TurboMix Attachment, 2.0 ml Permanently mounts to most existing Vortex-Genie 2 and Vortex-Genie 2T mixers converting them to a Disruptor Genie™.	S6004-2	1 unit	$172.00
FastPrep®-24 Instrument	S6005	1 unit	$7,625.00
HiPrep™ Attachment (Accomodates 48 x 2 ml tubes)	S6005-1	1 unit	$1,200.00
CryoPrep™ Attachment (Accomodates 24 x 2 ml tubes)	S6005-2	1 unit	$1,550.00
TeenPrep™ Attachment (Accomodates 12x 15 ml tubes)	S6005-3	1 unit	$1,200.00
BigPrep™ Attachment (Accomodates 2 x 50 ml tubes)	S6005-4	1 unit	$1,200.00

Download Data Sheet

FastPrep®-24 Instrument
S6005

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