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ZR
Soil Microbe DNA Kit™ (Free
Sample Kit Available)
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Simple, efficient isolation of humic-free DNA from soil
microbes in as little as 15 minutes including tough-to-lyse
bacteria, fungi, algae, and protozoa. |
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State-of-the-art,
ultra-high density BashingBeads™
are fracture resistant and chemically inert. |
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Can
be used with any bead mill, disrupter, or vortex
that can accommodate standard 2.0 ml tubes. |
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Omits
the use of organic denaturants as well as proteinases.
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SPECIFICATIONS
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Formats: Bead Beating, Spin Column. |
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Sample
Sources: DNA is isolated from bacteria, fungi, protozoa,
and algae in up to 0.25 g of soil or from fungal/bacterial cells
directly (see
protocol). |
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DNA
Purity: High-quality, humic-free DNA is eluted with
DNase/RNase-Free Water and is especially well suited for PCR.
Absorbance 260 nm/280 nm > 1.8. |
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DNA
Size Limits: > 1 kb. |
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DNA
Recovery: Typically, up to 25 µg total DNA is
eluted into 100 µl (25 µl minimum) DNA Elution Buffer
per sample. For DNA 75 bp to 10 kb, the recovery is 70-90%.
For DNA 11 kb to 23 kb the recovery is 50-70%. |
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Equipment:
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
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| DESCRIPTION
The ZR Soil Microbe DNA Kit™
is designed for the simple, rapid isolation of humic-free,
PCR-quality DNA from microbes in soil. The kit can be used
to successfully isolate DNA from tough-to-lyse bacteria,
fungi, protozoa, and algae that inhabit a range of samples
including clay, sandy, silty, peaty, chalky, and loamy soils.
Soil samples are added to a ZR BashingBead™
Lysis Tube where microbes are rapidly and efficiently
lysed by bead beating in a uniquely designed lysis buffer.
Our Fast-Spin column technology is then used to isolate
the DNA which is subsequently filtered to remove humic acids/polyphenols
that inhibit PCR. The entire procedure can be performed
in as little as 15 minutes, and there is no need for organic
denaturants or proteinases. The ZR Soil Microbe
DNA Kit™ can also be used to successfully
isolate DNA from cultured bacteria, fungi, and yeast. A
schematic of the procedure is shown to the right.
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The
ZR Soil Microbe DNA Kit™ can be used
to isolate high quality DNA from a variety of soil types which
yields robust products following PCR. Panel A:
Physical characteristics of sampled soils (1-5).
Panel B: Microbial, genomic DNA was isolated
from soil samples (1-5) using the ZR Soil Microbe DNA Kit™.
Approximately 10% of the eluted DNA was then separated in
a 0.8% (w/v) agarose/ethidium bromide gel. Panels
C and D show the results of PCR of microbial DNA
isolated from the samples with primers specific for prokaryotic
16S rRNA (C) or eukaryotic rRNA (D). In the figures, the 1
kb size marker (NEB) is as indicated and the arrows show the
prokaryotic 16S rRNA and eukaryotic rRNA PCR products. |
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DNA
isolated from Saccharomyces cerevisiae (strain TMY18) using
the ZR Soil Microbe DNA Kit™ is high-quality
and structurally intact. Equivalent amounts of yeast were
processed using the ZR Soil Microbe DNA Kit™ or the
kits from competitors M and E. Equal volumes of eluted DNA
were then analyzed in a 0.8% (w/v) agarose/ethidium bromide
gel. The size marker is a 1 kb ladder (NEB). |
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ORDERING INFORMATION:
| Products |
Description |
Protocol |
Cat
No. |
Size |
Price |
|
ZR Soil Microbe
DNA
Kit™
|
Simple,
rapid isolation of humic-free, PCR-quality DNA from microbes
in soil. |
|
D6001 |
50
preps. |
$182.00 |
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*Free
kit offer valid online only and while supplies last.
Limit 1 per U.S. customer only. |
| Components
Available Separately |
| ZR
Bashing Bead Lysis Tubes |
S6002-50 |
50
tubes |
$92.00 |
|
| Lysis
Solution |
D6001-3-40 |
40
ml |
$25.00 |
|
| Soil
DNA Binding Buffer |
D6001-1-100 |
100
ml |
$102.00 |
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| DNA
Pre-Wash Buffer |
D3004-5-15 |
15
ml |
$8.00 |
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| Soil
DNA Wash Buffer |
D6001-2-50 |
50
ml |
$15.00 |
|
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DNA Elution Buffer |
D3004-4-10 |
10
ml |
$12.00 |
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| Zymo-Spin
IV Filters
(Orange Tops) |
C1007-50 |
50
filters |
$50.00 |
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| Zymo-Spin
IV-HRC Filter
(Green Tops) |
C1010-50 |
50
filters |
$92.00 |
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| Zymo-Spin
IIC Columns |
C1011-50 |
50
columns |
$45.00 |
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| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
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| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
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| Lysis
Instruments Available Separately |
| Disruptor
Genie™ (120V w/ 2 ml tube holder assembly) |
S6001-120 |
1
unit |
$425.00 |
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| Disruptor
Genie™ (240V w/ 2 ml tube holder assembly) |
S6001-240 |
1
unit |
$425.00 |
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TurboMix
Attachment, 2.0 ml
Permanently mounts to most existing Vortex-Genie
2 and Vortex-Genie 2T mixers converting them to a Disruptor
Genie™. |
S6004-2 |
1
unit |
$172.00 |
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| FastPrep®-24
Instrument |
S6005 |
1
unit |
$7,625.00 |
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HiPrep™
Attachment
(Accomodates
48 x 2 ml tubes) |
S6005-1 |
1
unit |
$1,200.00 |
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CryoPrep™
Attachment
(Accomodates
24 x 2 ml tubes) |
S6005-2 |
1
unit |
$1,550.00 |
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TeenPrep™
Attachment
(Accomodates
12x 15 ml tubes) |
S6005-3 |
1
unit |
$1,200.00 |
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BigPrep™
Attachment
(Accomodates
2 x 50 ml tubes)
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S6005-4 |
1
unit |
$1,200.00 |
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