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Zymoclean Gel DNA Recovery Kit (Free Sample Kit Available)

 HIGHLIGHTS


Quick (15 minute) recovery of ultra-pure DNA from agarose gels.
Column design allows DNA to be eluted at high concentrations into minimal
volumes of solvent.
Eluted DNA is well suited for use in DNA ligation reactions, sequencing, DNA
labeling reactions, PCR, etc.
Omits the use of glass beads, organic denaturants, and proteinases.


SPECIFICATIONS  

Formats: Spin Column/Filtration Plate
DNA Purity: High quality, purified DNA is eluted with water which is especially well suited for sequencing, ligation reactions, andrestriction endonuclease digestions.
Minimal Elution Volume: ≥ 6 µl.
DNA Quantity: Typically, up to 5 µg total DNA per column or well. For 50 bp – 10 kb: 70-90% 11 – 23 kb: 50-70%.
Buffer Tolerance: TAE, TBE.
Equipment: Microcentrifuge (Zymoclean™) or centrifuge w/ microplate carriers (ZR-96 Zymoclean™).



DESCRIPTION

The Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit provide simple, rapid methods for the purification and concentration of high quality DNA from agarose gel slices. Both products feature a single buffer system that allows for efficient dissolving of agarose gel slices and the subsequent adsorption of DNA onto the matrices of the supplied Zymo-Spin I™ Columns or Zymo-Spin I-96™ Filtration Plate. The DNA is washed twice then eluted with a small volume of water. The entire DNA purification/concentration procedure typically takes about 15-20 minutes. The procedure is easy: simply add the specially formulated Agarose Dissolving Buffer (ADB™) to the gel slice containing your DNA sample, let dissolve, and transfer the mixture to the supplied column or filtration plate, then spin, wash, and elute the DNA. There is no need for organic denaturants or chloroform. Instead, the products utilize unique column and filtration plate technologies to yield high quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.


The Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit provide simple, rapid methods for the purification and concentration of high quality DNA from agarose gel slices. Both products feature a single buffer system that allows for efficient dissolving of agarose gel slices and the subsequent adsorption of DNA onto the matrices of the supplied Zymo-Spin I™ Columns or Zymo-Spin I-96™ Filtration Plate. The DNA is washed twice then eluted with a small volume of water. The entire DNA purification/concentration procedure typically takes about 15-20 minutes. The procedure is easy: simply add the specially formulated Agarose Dissolving Buffer (ADB™) to the gel slice containing your DNA sample, let dissolve, and transfer the mixture to the supplied column or filtration plate, then spin, wash, and elute the DNA. There is no need for organic denaturants or chloroform. Instead, the products utilize unique column and filtration plate technologies to yield high quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Gel DNA Recovery Kit and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly suited for use in DNA ligation reactions, sequencing, DNA labeling reactions, PCR, etc.



ORDERING INFORMATION:


Products
Protocol
Cat No.
Size
Price  
Zymoclean™ Gel DNA Recovery Kit
For 50 DNA preps.
D4001
1 kit
$65.00
Add to Cart
Zymoclean™ Gel DNA Recovery Kit
For 200 DNA preps.
D4002
1 kit
$247.00
Add to Cart
Try it for FREE!
*Free kit offer valid online only and while supplies last. Limit 1 per U.S. customer only.

Components Available Separately
ADB-Buffer™ (Agarose Dissolving Buffer) D4001-1-50 50 ml $28.00
Add to Cart
D4001-1-100 100 ml $56.00
Add to Cart
DNA Wash Buffer (concentrate) D4003-2-6 6 ml $7.00
Add to Cart
D4003-2-24 24 ml $28.00
Add to Cart
Zymo-Spin I Columns (uncapped) C1003-50 50 columns $35.00
Add to Cart
C1003-250 250 columns $152.00
Add to Cart
Collection Tubes   C1001-50 50 tubes $5.00
Add to Cart
C1001-500 500 tubes $30.00
Add to Cart
C1001-1000 1,000 tubes $50.00
Add to Cart
 

 







Zymoclean Spec Sheet - PDF
Download Data Sheet




DNA recovery using the Zymoclean Gel DNA Recovery Kit and blunt end ligation of the recovered DNA. Plasmid DNA fragments, digested by blunt ended enzyme PvuII, was recovered separately, then mixed and ligated at different times.
DNA recovery using the Zymoclean Gel DNA Recovery Kit and blunt end ligation of the recovered DNA. Plasmid DNA fragments, digested by blunt ended enzyme PvuII, was recovered separately, then mixed and ligated at different times.

Effectiveness of the Zymoclean Gel DNA Recovery Kit. Lanes: M: Ladder; 1-5: Excised and recovered bands from ladder.
Effectiveness of the Zymoclean Gel DNA Recovery Kit. Lanes: M: Ladder; 1-5: Excised and recovered bands from ladder.






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