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Zymoclean™
Gel DNA Recovery Kit
(Free
Sample Kit Available)
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Quick
(15 minute) recovery of ultra-pure DNA from agarose gels. |
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Column
design allows DNA to be eluted at high concentrations
into minimal
volumes of solvent. |
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Eluted
DNA is well suited for use in DNA ligation reactions,
sequencing, DNA
labeling reactions, PCR, etc. |
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Omits
the use of glass beads, organic denaturants, and proteinases.
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SPECIFICATIONS
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Formats: Spin Column/Filtration Plate |
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DNA
Purity: High quality, purified DNA is eluted with water
which is especially well suited for sequencing, ligation reactions,
andrestriction endonuclease digestions. |
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Minimal
Elution Volume: ≥ 6 µl. |
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DNA
Quantity: Typically, up to 5 µg total DNA per
column or well. For 50 bp – 10 kb: 70-90% 11 – 23
kb: 50-70%. |
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Buffer
Tolerance: TAE, TBE. |
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Equipment:
Microcentrifuge (Zymoclean™) or centrifuge w/ microplate
carriers (ZR-96 Zymoclean™). |
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DESCRIPTION
The Zymoclean™ Gel DNA Recovery Kit
and ZR-96 Zymoclean™ Gel DNA Recovery Kit
provide simple, rapid methods for the purification and concentration
of high quality DNA from agarose gel slices. Both products
feature a single buffer system that allows for efficient
dissolving of agarose gel slices and the subsequent adsorption
of DNA onto the matrices of the supplied Zymo-Spin I™
Columns or Zymo-Spin I-96™ Filtration Plate. The DNA
is washed twice then eluted with a small volume of water.
The entire DNA purification/concentration procedure typically
takes about 15-20 minutes. The procedure is easy: simply
add the specially formulated Agarose Dissolving Buffer (ADB™)
to the gel slice containing your DNA sample, let dissolve,
and transfer the mixture to the supplied column or filtration
plate, then spin, wash, and elute the DNA. There is no need
for organic denaturants or chloroform. Instead, the products
utilize unique column and filtration plate technologies
to yield high quality, purified DNA in just minutes. DNA
purified using the Zymoclean™ Gel DNA Recovery Kit
and ZR-96 Zymoclean™ Gel DNA Recovery Kit is perfectly
suited for use in DNA ligation reactions, sequencing, DNA
labeling reactions, PCR, etc.
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price |
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Zymoclean™
Gel DNA Recovery Kit
For 50 DNA preps.
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D4001 |
1
kit |
$65.00 |
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Zymoclean™
Gel DNA Recovery Kit
For 200 DNA preps.
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D4002 |
1
kit |
$247.00 |
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*Free
kit offer valid online only and while supplies last.
Limit 1 per U.S. customer only. |
| Components
Available Separately |
| ADB-Buffer™
(Agarose Dissolving Buffer) |
D4001-1-50 |
50
ml |
$28.00 |
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| D4001-1-100 |
100
ml |
$56.00 |
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| DNA
Wash Buffer (concentrate) |
D4003-2-6 |
6
ml |
$7.00 |
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| D4003-2-24 |
24
ml |
$28.00 |
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| Zymo-Spin
I Columns (uncapped) |
C1003-50 |
50
columns |
$35.00 |
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| C1003-250 |
250
columns |
$152.00 |
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| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
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| C1001-500 |
500
tubes |
$30.00 |
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| C1001-1000 |
1,000
tubes |
$50.00 |
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DNA
recovery using the Zymoclean Gel DNA Recovery Kit and blunt end
ligation of the recovered DNA. Plasmid DNA fragments, digested by
blunt ended enzyme PvuII, was recovered separately, then mixed and
ligated at different times. |
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Effectiveness
of the Zymoclean Gel DNA Recovery Kit. Lanes: M: Ladder; 1-5: Excised
and recovered bands from ladder. |
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