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Zyppy™
Plasmid
Maxiprep Kit
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Easy and fast procedure: lyse cells then vacuum, wash,
and elute DNA. |
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The fastest, simplest procedure for purifying the highest
quality endonuclease-free plasmid DNA. |
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Innovative
colored buffers* permit error-free visual identification
of complete bacterial cell lysis and neutralization.
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SPECIFICATIONS
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DNA Purity: Plasmid DNA is well suited for
ligation, sequencing, restriction endonuclease digestion, in
vitro transcription, and other sensitive applications requiring
pure DNA. Typical Abs.260/280 index is ≥ 1.8.
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Plasmid
DNA Yield: ≥ 500 µg
per preparation, depending on the plasmid copy number, culture
growth conditions, and strain of E. coli utilized. |
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Plasmid
DNA Size: Up to 25 kb. |
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Recovery
Volume: ≥ 25 ml. |
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Procedure:
Performed at room temperature (15-30°C) using a centrifuge
and/or a vacuum manifold. |
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DESCRIPTION
The Zyppy™ Plasmid Maxiprep Kit
employs a modified alkaline lysis method in conjunction
with spin-column purification to isolate high quality endonuclease-free
plasmid DNA in minutes. The purified DNA is suitable for
use in restriction endonuclease digestion, ligation, bacterial
transformation, PCR amplification, DNA sequencing and other
sensitive downstream applications.
The innovative colored buffers included in the kit permits
error-free visualization identification of complete bacterial
cell lysis and neutralization. Additionally, the uniquely
designed Zymo-Maxi Filter™ column
permits lysate clearing without centrifugation while the
high capacity DNA-binding Zymo-Spin™ VI column
allows for low 2–3 ml elution volumes, eliminating
the eluted plasmid DNA precipitation and resuspension steps
common to other column-based maxipreparation procedures.
The Zyppy™ Plasmid Maxiprep Kit is
designed for use with a centrifuge, a vacuum manifold, or
a combination of both procedures and provides the greatest
flexibility in large scale plasmid DNA purification from
E. coli. An overview of the purification procedure
is shown below.
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ORDERING INFORMATION
| Products |
Protocol |
Cat
No. |
Size |
Price |
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Zyppy™
Plasmid Maxiprep Kit
10 preps.
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D4027 |
1
kit |
$102.00 |
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Zyppy™
Plasmid Maxiprep Kit
20 preps.
|
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D4028 |
1
kit |
$202.00 |
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| Components
Available Separately |
|
P1 Buffer (Red) |
D4027-1-160 |
160
ml |
$22.00 |
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| D4027-1-320 |
320
ml |
$38.00 |
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| P2
Buffer
(Green) |
D4027-2-160 |
160
ml |
$22.00 |
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| D4027-2-320 |
320
ml |
$38.00 |
|
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P3 Buffer
(Yellow) |
D4027-3-220 |
220
ml |
$38.00 |
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| D4027-3-440 |
440
ml |
$70.00 |
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| Endo-Wash
Buffer |
D4036-3-120 |
120
ml |
$42.00 |
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| D4036-3-240 |
240
ml |
$75.00 |
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| Zyppy
Wash Buffer
(concentrate) |
D4036-4-24 |
24
ml |
$30.00 |
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| D4036-4-48 |
48
ml |
$42.00 |
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| Zyppy™
Elution Buffer |
D4036-5-30 |
30
ml |
$35.00 |
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| D4036-5-60 |
60
ml |
$65.00 |
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| RNase
A |
E1008-2 |
2
mg |
$20.00 |
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| E1008-8 |
8
mg |
$30.00 |
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| Zymo-Maxi
Filter™ Columns |
C1017-10 |
10
pk |
$30.00 |
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| Zymo-Spin
VI Columns |
C1013-10 |
10
pk |
$30.00 |
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