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| Zyppy™
Plasmid
Miniprep Kit |
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Promotion! |
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Pellet-Free™. No conventional cell-pelleting or
resuspension steps required. |
| • |
The fastest, simplest procedure for purifying the highest
quality endotoxin-free plasmid DNA. |
| • |
Innovative
colored buffers for error-free visual identification of
complete bacterial cell lysis and neutralization.
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SPECIFICATIONS
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DNA Purity: Plasmid DNA which is well suited
for ligation, sequencing, restriction endonuclease digestion,
in vitro transcription, and other sensitive applications
requiring pure DNA. Typical Abs.260/280 index is
≥ 1.8. |
| • |
Plasmid
DNA Yield: Up to 25 µg
per preparation, depending on the plasmid copy number, culture
growth conditions, and strain of E. coli utilized. |
| • |
Plasmid
DNA Size: Up to 25 kb. |
| • |
Recovery
Volume: ≥ 30 µl. |
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Procedure:
Performed at room temperature (15-30°C). |
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DESCRIPTION
The Zyppy™ Plasmid Miniprep Kit
features a Pellet-Free™
modified alkaline lysis method that bypasses bacterial culture
centrifugation and resuspension steps common to classical
plasmid preparation procedures. Simply add the uniquely
formulated 7X Lysis Buffer directly to
your bacterial culture, neutralize, then purify using our
Fast-Spin column technology (alternatively, the samples
may be processed by the classical centrifugation method).
Additionally, the innovative colored buffers included in
the kit permit error-free visualization identification of
complete bacterial cell lysis and neutralization.
Our Zyppy™ Plasmid Miniprep Kit is
the fastest and simplest method available to efficiently
separate plasmid DNA from E. coli. The plasmid
DNA is of the highest quality, is endotoxin-free, and is
well suited for use in bacterial transformation, restriction
endonuclease digestion (below), DNA ligation, PCR, transcription,
sequencing, and other sensitive downstream applications.
An overview of the purification procedure is shown to the
upper right.
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ORDERING INFORMATION:
| Products |
Protocol |
Cat
No. |
Size |
Price** |
|
Zyppy™
Plasmid Miniprep Kit
Reagents provided are for 50 preps.
|
|
D4036 |
1
kit |
$52.00 |
|
Zyppy™
Plasmid Miniprep Kit
Reagents provided are for 100 preps.
|
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D4019 |
1
kit |
$95.00 |
|
Zyppy™
Plasmid Miniprep Kit
Reagents provided are for 400 preps.
|
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D4020 |
1
kit |
$320.00 |
|
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Please
use coupon code
ZRC150 to receive a 50%
discount. Offer
valid for US customers only.
|
| |
|
| Inform
a colleague about this exciting opportunity.
Free sample offer valid for US customers only. |
| Components
Available Separately |
| 7X
Lysis Buffer
(Blue) |
D4036-1-6 |
6
ml |
$12.00 |
|
| D4036-1-12 |
12
ml |
$32.00 |
|
| D4036-1-30 |
30
ml |
$40.00 |
|
| D4036-1-48 |
48
ml |
$60.00 |
|
| D4036-1-60 |
60
ml |
$70.00 |
|
| Neutralization
Buffer
(Yellow) |
D4036-2-20 |
20
ml |
$10.00 |
|
| D4036-2-40 |
40
ml |
$30.00 |
|
| D4036-2-100 |
100
ml |
$70.00 |
|
| D4036-2-160 |
160
ml |
$110.00 |
|
| D4036-2-200 |
200
ml |
$120.00 |
|
| Endo-Wash
Buffer |
D4036-3-15 |
15
ml |
$8.00 |
|
| D4036-3-30 |
30
ml |
$15.00 |
|
| D4036-3-60 |
60
ml |
$30.00 |
|
| D4036-3-120 |
120
ml |
$42.00 |
|
| D4036-3-240 |
240
ml |
$75.00 |
|
| Zyppy
Wash Buffer
(concentrate) |
D4036-4-6 |
6
ml |
$8.00 |
|
| D4036-4-12 |
12
ml |
$15.00 |
|
| D4036-4-24 |
24
ml |
$30.00 |
|
| D4036-4-48 |
48
ml |
$42.00 |
|
| Zyppy™
Elution Buffer |
D4036-5-5 |
5
ml |
$8.00 |
|
| D4036-5-10 |
10
ml |
$15.00 |
|
| D4036-5-20 |
20
ml |
$22.00 |
|
| D4036-5-30 |
30
ml |
$35.00 |
|
| D4036-5-60 |
60
ml |
$65.00 |
|
| RNase
A |
E1008-2 |
2
mg |
$20.00 |
|
| E1008-8 |
8
mg |
$30.00 |
|
| Zymo-Spin
IIN Columns |
C1019-50 |
50
columns |
$35.00 |
|
| C1019-250 |
250
columns |
$152.00 |
|
| Collection
Tubes |
C1001-50 |
50
tubes |
$5.00 |
|
| C1001-500 |
500
tubes |
$30.00 |
|
| C1001-1000 |
1,000
tubes |
$50.00 |
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Review
Snyopsis |
A
very well thought out product. It demonstrates sufficient improvement
over the kit I’ve been using for me to switch.
- Michael Campa, PhD
Click here
to read the full article |
Download
Data Sheet
|
