Zymo Research - Mini RNA Isolation II Kit

Mini RNA Isolation II Kit™

HIGHLIGHT

•	Allows for the isolation of total RNA from a wide range of cell types using Fast- Spin column technology.
•	Simply and reliably isolates total RNA from cells (1x10²-5x10⁶ cells) and from tissue samples (up to 20 mg) in about 10 minutes.
•	Single buffer system is used for both liquid and tissue samples that inactivates nucleases more efficiently than classical methods.
•	Eluted RNA is suitable for use in RNA-based procedures.
•	Omits the use of organic denaturants as well as proteinases.

SPECIFICATIONS

•	Format: Spin Column.
•	RNA Recovery: Typically, up to 25 µg RNA is eluted into ≥ 50 µl of RNase-free water allowing for a highly concentrated sample. Recovery is approximately 80-95% of input RNA.
•	RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C.
•	RNA Size Limits: From 200 bases up to 35 kb.
•	Sample Sources: Cells from culture or solid tissue.

DESCRIPTION

The Mini RNA Isolation II Kit™ is an innovative product designed for the easy, reliable, and rapid isolation of total RNA from cultured cells or solid tissue samples. The procedure combines a unique, single step RNA extraction/binding buffer with Zymo Research’s Fast-Spin column technology to yield high quality, purified RNA in about 10 minutes. The Mini RNA Isolation II Kit™ allows for the efficient recovery of total RNA from cells (1x10²-5x10⁶ ) or solid tissue (up to 20 mg). The method is easy: simply add the provided ZR RNA Buffer™ to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ Columns. The result is high quality, purified RNA that is suitable for subsequent RNA-based analyses including RT-PCR.

ORDERING INFORMATION:

Products	Protocol	Cat No.	Size	Price
Mini RNA Isolation II Kit™ For 50 sample processes (Supplied with capped columns)	PDF	R1030	1 kit	$122.00
Mini RNA Isolation II Kit™ For 200 sample processes (Supplied with capped columns)	PDF	R1031	1 kit	$398.00
Mini RNA Isolation II Kit™ For 50 sample processes (Supplied with uncapped columns)	PDF	R1032	1 kit	$118.00
Mini RNA Isolation II Kit™ For 200 sample processes (Supplied with uncapped columns)	PDF	R1033	1 kit	$392.00

Components Available Separately
ZR RNA Buffer	R1020-1-50	50 ml	$68.00
ZR RNA Buffer	R1020-1-100	100 ml	$122.00
RNA Wash Buffer	R1003-3-2.4	2.4 ml	$9.00
	R1003-3-6	6 ml	$15.00
	R1003-3-12	12 ml	$26.00
	R1003-3-24	24 ml	$52.00
	R1003-3-48	48 ml	$92.00
Dnase/RNase Free water	W1001-1	1 ml	$2.00
	W1001-4	4 ml	$3.00
	W1001-6	6 ml	$4.00
	W1001-10	10 ml	$6.00
Zymo-Spin III Columns (uncapped)	C1005-50	50 columns	$40.00
Zymo-Spin III Columns (uncapped)	C1005-250	250 columns	$190.00
Zymo-Spin IIIC Columns™ (capped)	C1006-50	50 columns	$42.00
Zymo-Spin IIIC Columns™ (capped)	C1006-250	250 columns	$198.00
Collection Tubes	C1001-50	50 tubes	$5.00
	C1001-500	500 tubes	$30.00
	C1001-1000	1,000 tubes	$50.00