Zymo Research - ZR Urine RNA Isolation Kit

ZR Urine RNA Isolation Kit™

HIGHLIGHTS

•	Quick, simple and reliable recovery of purified RNA from cells in urine samples.
•	Ideal for recovering total RNA from large volume liquid samples that contain a low concentration of cells.
•	Column design allows RNA to be eluted at high concentrations into minimal volumes of the supplied elution buffer or RNase-free water.
•	Eluted RNA is ideal for use in RT-PCR.
•	Omits the use of organic denaturants as well as proteinases.

SPECIFICATIONS

•	Format: Filtration, Spin Column.
•	RNA Storage: Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C.
•	Sample Sources: Urine.
•	RNA Recovery: Typically, 0.2-3.0 µg RNA per 30 ml urine sample. The RNA binding capacity of the supplied columns is 5 µg.
•	Sample Sizes: 30 ml per standard reaction, but can be increased/decreased proportionally.
•	Equipment: Syringe, microcentrifuge.

DESCRIPTION

Zymo Research’s ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated RNA Extraction Buffer Plus™ reagent. The collected lysate can then be used immediately or at a later time following transportation and/or storage. One-step RNA isolation occurs via matrix adsorption using Zymo-Spin IC™ Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit™ results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.

Zymo Research’s ZR Urine RNA Isolation Kit™ is an innovative product designed for the easy, reliable and rapid isolation of total RNA from cells in urine samples. The product enables isolation of cells from urine using a syringe and a uniquely-designed syringe filter. Following separation, cells are lysed and total RNA stabilized using a specially formulated RNA Extraction Buffer Plus™ reagent. The collected lysate can then be used immediately or at a later time following transportation and/or storage. One-step RNA isolation occurs via matrix adsorption using Zymo-Spin IC™ Columns. The RNA isolation procedure is simple and can be performed in less than 5 minutes. Use of the ZR Urine RNA Isolation Kit™ results in the isolation of high-quality, total RNA from urine samples that is suitable for subsequent analyses of gene expression that include RT-PCR.

ORDERING INFORMATION:

Products	Protocol	Cat No.	Size	Price
Urine RNA Isolation Kit™-20 For 20 sample preps.	PDF	R1038	1 kit	$105.00
Urine RNA Isolation Kit™-50 For 50 sample preps.	PDF	R1039	1 kit	$241.00

Components Available Separately
RNA Extraction Buffer Plus	R1038-1-20	20 ml	$27.00
	R1038-1-50	50 ml	$69.00
RNA Wash Buffer (concentrate)	R1003-3-2.4	2.4 ml	$9.00
	R1003-3-6	6 ml	$15.00
	R1003-3-12	12 ml	$26.00
	R1003-3-24	24 ml	$52.00
	R1003-3-48	48 ml	$92.00
RNA Elution Buffer	R1003-4-1	1 ml	$15.00
Zymo Spin IC Columns (capped)	C1004-50	50 columns	$46.00
	C1004-250	250 columns	$197.00
ZRC GF Filter	C1009-20	20 filters	$44.00
	C1009-50	50 filters	$110.00
Collection Tubes	C1001-50	50 tubes	$5.00
	C1001-500	500 tubes	$30.00
	C1001-1000	1,000 tubes	$50.00