Zymo Research - Yeastar RNA Kit

YeaStar™ RNA Kit

HIGHLIGHTS

•	Recovery of purified RNA from a wide range of fungus species using Fast-Spin column technology.
•	Eluted RNA is suitable for use in RT-PCR or other RNA-based procedures.
•	Omits the use of organic denaturants as well as the use of glass beads.

SPECIFICATIONS

•	RNA Purity: High quality, purified total RNA is recovered in the supplied RNase-free water.
•	RNA Recovery: Typically, RNA is eluted into 60 µl RNase-free water. The provided columns have an RNA binding capacity of 25 µg.
•	Sample Sources: Fungi susceptible to yeast lytic enzyme lysis.
•	Sample Sizes: 1 to 1.5 ml liquid culture.
•	Stability of Product Reagents: Integrity of kit components is guaranteed for up to one year from date of purchase.
•	Quality Control: Reagents are routinely tested on a lot-to-lot basis to ensure they provide maximal performance and reliability.

DESCRIPTION

The YeaStar RNA Kit™ provides all the necessary reagents for RNA isolation from a broad spectrum of fungi including: Aspergillus fumigatus, Aspergillus nidulans, Aspergillus nivens var. aureus, Candida albicans, Pichia pastoris, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Generally, the kit can be used for the purification of high-quality, total RNA from any fungus that can be lysed by yeast lytic enzyme. The kit facilitates the purification of 10-25 µg of total RNA from 1- 1.5 ml of cultured cells using Zymo Research’s Fast-Spin column technology.

ORDERING INFORMATION:

Products	Protocol	Cat No.	Size	Price
YeaStar RNA Kit	PDF	R1001 (uncapped columns)	1 kit	$104.00
YeaStar RNA Kit	PDF	R1002 (capped columns)	1 kit	$112.00

Components Available Separately
YR Digestion Buffer	R1001-1	3.2 ml	$20.00
YR Lysis Buffer	R1001-2	6.4 ml	$20.00
RNA Wash Buffer	R1003-3-2.4	2.4 ml	$9.00
	R1003-3-6	6 ml	$15.00
	R1003-3-12	12 ml	$26.00
	R1003-3-24	24 ml	$52.00
	R1003-3-48	48 ml	$92.00
DNase/RNase Free water	W1001-1	1 ml	$2.00
	W1001-4	4 ml	$3.00
	W1001-6	6 ml	$4.00
	W1001-10	10 ml	$6.00
Lyophilized Zymolyase	E1004	1000 units	$52.00
Lyophilized Zymolyase	E1005	2000 units	$86.00