About DNA Degradase Plus™
DNA Degradase Plus™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus™ is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
Specifications
| Format | Provided in solution (5 units/µl) w/ 10X Reaction Buffer | |||||||
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| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be ≤ -70°C. | |||||||
| Unit Definition | One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C. | |||||||
| Reaction Conditions | DNA Degradase Plus™ in 1X DNA Degradase™ Reaction Buffer. Incubate reaction mixtures at 37°C for ≥1 hour. | |||||||
| Inactivation | Heat-inactivate at 70°C for 20 minutes. | |||||||
| Standard Reaction Setup | The setup (below) is an example of a typical DNA Degradase Plus™ reaction in a 25 µl final volume.
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DNA Degradase Plus™ efficiently degrades DNA: 1 or 5 µg of λ DNA incubated with 5 U of DNA Degradase Plus™ in 25 µl reaction volume at 37°C for 1 hour. "M" is a 1 kb DNA ladder (Zymo Research).
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