About DNA-Free RNA Kit™
The DNA-Free RNA Kit™ provides a simple and reliable method for the rapid preparation of up to ~5 µg (per prep.) of high-quality RT-PCR-ready, DNA-free RNA. The kit is provided with high fidelity DNase I for complete DNA removal. Purification of the RNA is easy: simply treat your RNA sample with DNase I, add the binding buffer, adjust the conditions by adding ethanol, and then bind, wash, and elute the pure RNA from the provided Zymo-Spin™ IC Column. RNA ≥ 17 bases can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 20 min |
| Sample Sources | RNA fragments (≥17 nucleotides), labeled RNA fragments, in vitro transcription products, etc. |
| RNA Purity | High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery | Typically, up to 5 µg of RNA is eluted into as little as 6-10 µl RNase-free water allowing for a highly concentrated sample. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| Equipment Needed | Microcentrifuge |
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Purification of RNA samples containing genomic and plasmid DNA treated with the DNA-Free RNA Kit™. (1) RNA/DNA sample purified without prior DNase treatment. (2) DNase treated, purified RNA sample. M is a 1 kb DNA Marker (Zymo Research).
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