About RNA Clean & Concentrator™-100
The RNA Clean & Concentrator™-100 provides a simple and reliable method for the rapid preparation of high-quality RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: just add the binding buffer to your sample, adjust the conditions for binding by adding ethanol, wash, and elute the concentrated RNA. RNA ≥ 17 bases can be safely treated and recovered using this kit. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 10 min |
| Sample Sources | Modified, labeled, impure RNA, DNase treated RNA, in vitro transcription products, RNA from aqueous phase following acid phenol extraction methods (e.g., TRI Reagent®). |
| RNA Purity | High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery | Up to 125 µg of RNA can be eluted into ≥100 µl RNase-free water allowing for a highly concentrated sample. |
| Size Limit | RNA ≥17 nucleotides |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is recommended for prolonged storage. |
| Equipment Needed | Vacuum manifold, microcentrifuge |
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