About Direct-zol™ RNA MiniPrep
The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 μg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product.
The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
The entire procedure typically takes about 5-10 minutes.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 10 min |
| Equipment | Microcentrifuge |
| Sample Sources | Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI-Reagent®, TRIzol®, or similar. |
| RNA Purity | High quality RNA (A260/A280 >1.8, A260/A230 >1.8) is recovered. |
| RNA Recovery | The RNA binding capacity of the supplied Zymo-Spin™ IIC Column is ~50 μg with a minimal elution volume of 25 μl. |
| Compatibility | TRI-Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure, RNA-Bee or similar acid-guanidinium-phenol based solutions. |
| RNA Storage | ≤-70 ºC. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage. |
| RNA Size Limits | RNAs ≥17 nucleotides. |
High quality broad range RNA is purified with the Direct-zol™ RNA MiniPrep. (A) DNA-free RNA purified from human epithelial cells using the Direct-zol™ RNA MiniPrep compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). (B) Small RNAs are effectively recovered with the Direct-zol™ procedure while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown)
Viral RNA is detected with high sensitivity following the Direct-zol™ isolation method. The Direct-zol™ method significantly improves the detection of West Nile virus when compared to the conventional phaseseparation method. The RT-qPCR data show ΔCt = 5 (average of two independent experiments). RNA was isolated from cell-free samples inactivated using the TRIReagent®.
