About ZR RNA MicroPrep™
The ZR RNA MicroPrep™ provides a quick method for high quality total RNA isolation from cells, needle biopsies, and tissue. The product isolates both large and small RNA species without the use of reducing agents or phenol. Small RNAs (e.g., tRNAs, microRNAs) can be recovered with a simple adjustment of the RNA isolation protocol. Eluted RNA is suitable for use in RT-PCR and other RNA-based procedures.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 15 min (does not account for in-column DNase I treatment (~20 min)) |
| Equipment | Microcentrifuge |
| Sample Sources | Cells from culture or small amounts of solid tissue. |
| Storage | ≤-70ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
| RNA Purity | High quality RNA (A260/A280>1.8, A260/A230>1.8) is recovered. |
| RNA Recovery | RNA can be eluted into small volumes, ≥ 6 µl allowing for a highly concentrated sample. Maximum RNA binding capacity of provided column is ~5 µg. |
| Sample Size | 101 - 105 cells in suspension or solid form. |
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Recovery of small RNA. Total RNA isolated using the ZR RNA MicroPrep™ was resolved in an agarose gel (2-4) and small RNAs from the same sample were also resolved in a native polyacrylamide gel (6-7). Input = 105 yeast cells spiked with 1 µg ZR small-RNA™ Ladder (Zymo Research)
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PCR amplification of ß-actin transcript post-RT (353 bp fragment shown): Total RNA from human epithelial cells (HCT 116) was isolated using the ZR RNA MicroPrep™.
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