About ZR Fungal/Bacterial RNA MiniPrep™
The ZR Fungal/Bacterial RNA MiniPrep™ provides for rapid isolation of total RNA from pelleted tough-to-lyse bacterial (e.g., Gram-positive), yeast, and/or fungal cells. The kit employs ultra-high density BashingBeads™ for sample homogenization and a robust buffer system for total RNA purification (small RNAs included). Using Fast-Spin column technology, the RNA is eluted into volumes as little as 25 µl and suitable for subsequent procedures including RT-PCR. The entire RNA isolation procedure typically takes less than 15 minutes.
Specifications
| Format | Spin Column |
|---|---|
| DNA Recovery | 15 min |
| Equipment | Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional). |
| Sample Sources | Fresh or frozen pelleted cells (bacteria, yeast, fungi). |
| RNA Purity | High quality RNA (A260/A280>1.8, A260/A230>1.8) recovered. |
| RNA Recovery | Up to 25 µg RNA can be eluted into ≥ 25 µl, allowing for a highly concentrated sample. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Total RNA was isolated from equal amounts of pelleted E. coli cells containing plasmid DNA (pGEM®) using the ZR Fungal/Bacterial RNA MicroPrep™ or kit from Supplier A. The samples were resolved in a 2% (w/v) agarose gel. RNA Millenium Markers (Ambion) and ZR 1 kb DNA Marker (Zymo Research) were used.
* = genomic (> 10 kb) and plasmid (> 3 kb) DNA contamination; |
