About Pinpoint™ Slide RNA Isolation System I
The Pinpoint™ Slide RNA Isolation System I is an innovative product for the isolation of RNA from any targeted area of fresh tissue sectioned onto a glass slide. The system combines powerful Pinpoint™ tissue sampling methodology, a unique single-step RNA extraction/binding buffer, and Fast-Spin column purification technology to yield high quality RNA. Unlike current UV-based methods, this product makes isolation of tissue RNA simple and quick. No expensive specialized equipment is needed. Eluted RNA is well suited for subsequent RNA analyses including RT-PCR.
Specifications
| Format | Spin Column. |
|---|---|
| Processing Time | 1.5 hr |
| Equipment | Microcentrifuge |
| Sample Sources | Cells from tissue sections on glass slides. |
| RNA Recovery | Typically, up to 5 µg RNA is eluted into ≥ 8 µl RNase/DNase-free water allowing for a highly concentrated sample. |
| RNA Storage | Recommended that 1 U/10 µl RNase inhibitor be added to the RNA prior to storage at -70°C. |
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RT-PCR of RNA recovered from human tissue using the Pinpoint™ RNA Isolation System. Amplicons (in duplicate) are from A) a human ß-actin transcript; B) an arbitrary human transcript from Chromosome 3. M is 100 bp DNA Marker (Zymo Research).
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