About ZR Tissue & Insect RNA MicroPrep™
The ZR Tissue & Insect RNA MicroPrep™ provides for rapid isolation of total RNA from various tissue samples, insect, and arthropod specimens (e.g., mosquitoes, bees, lice, ticks, Drosophila melanogaster). Soft and solid mammalian tissues can also be processed with this kit. The product employs ultra-high density BashingBeads™ for sample homogenization and a robust buffer system delivering total RNA purification (small RNAs included). The Zymo-Spin™ IIIC Column allows for high-capacity DNA elimination and the Zymo-Spin™ IC Column is used for purifying the total RNA. RNA eluted in DNase/RNase-free water is suitable for subsequent procedures including RT-PCR.
Specifications
| Format | Spin Column |
|---|---|
| Processing Time | 15 min |
| Equipment | Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional). |
| Sample Sources | Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). |
| RNA Purity | High quality RNA (A260/A280>1.8, A260/A230>1.8) recovered. |
| RNA Recovery | Up to 5 µg RNA can be eluted into ≥ 6 µl, allowing for a highly concentrated sample. |
| RNA Storage | RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Analysis of ZR Tissue & Insect RNA MicroPrep™. Isolation of total RNA from n=2 Drosophila sp. individuals was performed in duplicate (lanes 1 and 2). Samples were processed (2 x 30 sec at 6 m/s) using a FastPrep®-24 Instrument (MP Biomedicals) and resolved alongside (lane M) RNA MilleniumTM Markers (Ambion) in a 1% (w/v) non-denaturing agarose gel.
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