About ZR Viral RNA Kit™
The ZR Viral RNA Kit™ is designed for the rapid isolation of high-quality viral RNA from a wide range of biological sources. The kit can be used to isolate viral RNA from cell-free body fluids and cellular suspensions at concentrations ≤ 105 cells/ml. The product has been rigorously tested and used to isolate viral RNA from samples containing enteroviruses, rhinoviruses, coronaviruses, HIV, HCV, influenza A virus, flaviviruses, measles virus, parainfluenza virus, and parvovirus (an ssDNA virus). Eluted RNA is suitable for use in subsequent procedures, including RT-PCR.
Specifications
| Format | Spin Column |
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| Processing Time | 5 min |
| Equipment | Microcentrifuge |
| Sample Sources | Serum, plasma, tissue, or cells derived from humans, mice, rabbits, etc. |
| RNA Purity | High quality RNA is recovered in the supplied RNase-free water. |
| RNA Recovery | Typically, up to 5 µg total RNA can be eluted into ≥ 6 µl water or elution buffer. |
| RNA Size Limits | From 200 bases up to 35 kb. |
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RT-PCR amplification of enterovirus cDNA. Human serum was spiked with different amounts of infectious enterovirus, then viral RNA was extracted using the ZR Viral RNA Kit™. The eluted RNA was used for one-tube RT-PCR amplification of a 230 bp amplicon. M is a 100 bp DNA Marker (Zymo Research)
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Viral RNA was isolated from liquid samples using the ZR Viral RNA Kit™. Isolated viral RNA was reverse transcribed/amplified using a coupled RT-real-time PCR system (Zymo Research). Ct values for measles and influenza type A (FluA) were 23.05 (diamonds), 24.56 (triangles), respectively.
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