About ZR-Duet™ DNA/RNA MiniPrep (Plus)
(soft, lipid, fibrous, FFPE)
gDNA removal column
DNA/RNA Shield (for sample storage)
|gDNA removal column|
The ZR-Duet™ DNA/RNA kits provide a quick method for the isolation of high quality genomic DNA and total RNA from cells, tissue, blood, and biological fluids. The kits isolate both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs and microRNAs ≥17nt are also recovered – no extra steps required!
|Sample Sources||Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and samples in DNA/RNA Shield™.|
|Storage||DNA and RNA is eluted with DNase/RNase-free water and can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.|
|Sample Size||Up to 107 cells in suspension or up to 50 mg of tissue.|
|Purity||High quality genomic DNA and total RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.|
|Size Limit||Capable of recovering genomic DNA ≥40 kb. Total RNA ≥17 nt can be recovered.|
|Equipment Needed||Microcentrifuge, vortex, and 55°C heat block, water bath or incubator.|
|Recovery||The DNA/RNA binding capacity of the included columns is 100 µg.|
|Sample Preservation||DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.|
Genomic DNA (lane 1, 2) and total RNA (lane 3, 4) isolated from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep.
PCR amplification of β-actin transcript (353 bp fragment shown) following DNA and RNA isolation from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep: PCR positive control (DNA template; lane 1, 2, 3), PCR negative control (RNA template; lane 4, 5, 6), RT-PCR (lane 7, 8, 9).
Nucleic acids were isolated from viral particle enriched wastewater using the ZR Viral DNA/RNA Kit™ and then sequenced for metagenomics analysis on Illumina platforms. Based on alignments and a nonuniversal genetic code, results suggest the first described RNA viruses of ciliates.