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ZR-Duet™ DNA/RNA MiniPrep (Plus)

Efficient isolation and separation of DNA and RNA from any cells, tissue, blood, and biological fluids.
High quality DNA and DNA-free RNA is ready for use in any downstream application. DNase I included (Plus kit only)

Product Size Catalog # Price Qty
NEW ZR-Duet ™ DNA/RNA MiniPrep Plus 50 Preps D7003
$399.00
ZR-Duet™ DNA/RNA MiniPrep 50 Preps D7001
$325.00

About ZR-Duet™ DNA/RNA MiniPrep (Plus)

MiniPrep Plus MiniPrep
Cells
Any tissue
(soft, lipid, fibrous, FFPE)
Whole blood
Cells
Soft tissue
Included
DNase I
gDNA removal column
Proteinase K
DNA/RNA Shield (for sample storage)
gDNA removal column

The ZR-Duet™ DNA/RNA kits provide a quick method for the isolation of high quality genomic DNA and total RNA from cells, tissue, blood, and biological fluids. The kits isolate both genomic DNA and a broad range of RNA species without the use of phenol. Small RNAs and microRNAs ≥17nt are also recovered – no extra steps required!


Sample Sources Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and samples in DNA/RNA Shield™.
Storage DNA and RNA is eluted with DNase/RNase-free water and can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.
Sample Size Up to 107 cells in suspension or up to 50 mg of tissue.
Purity High quality genomic DNA and total RNA (A260/A280 >1.8, A260/A230 >1.8) are recovered.
Size Limit Capable of recovering genomic DNA ≥40 kb. Total RNA ≥17 nt can be recovered.
Equipment Needed Microcentrifuge, vortex, and 55°C heat block, water bath or incubator.
Recovery The DNA/RNA binding capacity of the included columns is 100 µg.
Sample Preservation DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.

Genomic DNA (lane 1, 2) and total RNA (lane 3, 4) isolated from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep.
 

PCR amplification of β-actin transcript (353 bp fragment shown) following DNA and RNA isolation from human epithelial cells (HCT 116) with the ZR-Duet™ DNA/RNA MiniPrep: PCR positive control (DNA template; lane 1, 2, 3), PCR negative control (RNA template; lane 4, 5, 6), RT-PCR (lane 7, 8, 9).
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Featured Citations

Nucleic acids were isolated from viral particle enriched wastewater using the ZR Viral DNA/RNA Kit™ and then sequenced for metagenomics analysis on Illumina platforms. Based on alignments and a nonuniversal genetic code, results suggest the first described RNA viruses of ciliates.

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