RNA Clean & Concentrator™-5

Quick, 5 minute recovery of ultra pure RNA (≥17 nt) from enzymatic reactions and other samples.
High quality RNA eluted in ≥6 µl is ready for library prep, reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
RNA Clean & Concentrator™- 5 50 Preps. R1015
RNA Clean & Concentrator™- 5 200 Preps. R1016

About RNA Clean & Concentrator™-5

The RNA Clean & Concentrator™-5 provides a simple and reliable method for the rapid preparation of high-quality RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: just add the binding buffer to your sample, adjust the conditions for binding by adding ethanol, wash, and elute the concentrated RNA. RNA ≥ 17 bases can be safely treated and recovered using these kits. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.

Format Spin Column
Processing Time 5 min
Equipment Microcentrifuge
Sample Sources RNA fragments (≥ 17 nucleotides): DNase treated RNA, labeled RNA fragments, in vitro transcription products, etc.
Storage RNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.
RNA Purity High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.
RNA Recovery Typically, up to 10 µg of RNA is eluted into as little as 6-10 µl RNase-free water allowing for a highly concentrated sample.

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"We used the RNA Clean & Concentrator columns during our high-throughput sequencing library preparation. We used them to remove enzymes and buffers between each enzymatic reaction. We also used the >200nt protocol to remove unligated cloning linkers."

“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”
Anupma C.
“Great yield from a tiny amount of genomic RNA.”
Andrew H. (University of British Columbia)
“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
Caroline S. (Cornell University)
“It’s [an] excellent kit to work with tissues with high poly-phenols and acids.”
Arun D. (University of Missouri)
“The Zymo-Spin IC column is convenient.”
Lihua D. (National Institutes of Health)
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