RNA Clean & Concentrator™-5

Quick, 5 minute recovery of ultra pure RNA (≥17 nt) from enzymatic reactions, aqueous phase (following Trizol® extraction) and other sources.
High-quality RNA eluted in ≥6 μl is ready for reverse transcription, microarray, sequencing, etc.

Product Size Catalog # Price Qty
RNA Clean & Concentrator™-5 50 Preps R1015
$134.00
RNA Clean & Concentrator™-5 (supplied with DNase I) 50 Preps R1013
$162.00
RNA Clean & Concentrator™-5 200 Preps R1016
$462.00
RNA Clean & Concentrator™-5 (supplied with DNase I) 200 Preps R1014
$562.00

About RNA Clean & Concentrator™-5

RNA Clean & Concentrator-5 provides a simple and reliable method for the rapid preparation of up to 10 µg of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA.  This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).

The procedure is easy:  Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA.  The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥6 µl of RNase-free water.  The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

The entire procedure typically takes about 5 minutes.


Sample Sources DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol®/chloroform or similar extraction.
RNA Purity High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for reverse transcription, microarray, sequencing etc.
RNA Recovery Up to 10 μg RNA can be eluted into as little as ≥6 μl RNase-free water allowing for a highly concentrated sample.
RNA Storage RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is optional but highly recommended for prolonged storage.
Equipment Needed Microcentrifuge
RNA Size Limits From 17 nt to ~23 kb.

    
 
Concentration of diluted RNA samples.
RNA was eluted with 20 μl RNase-free water (n = 3, total input = 1 μg RNA).
Purification of small and large RNAs into separate fractions.
RNA Clean & Concentrator™ allows for purification of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).

Click Here to download the protocol
For specific notes and additional information, please see the product protocol PDF.
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Featured Citations

"We used the RNA Clean & Concentrator columns during our high-throughput sequencing library preparation. We used them to remove enzymes and buffers between each enzymatic reaction. We also used the >200nt protocol to remove unligated cloning linkers."

“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”
Anupma C.
“Great yield from a tiny amount of genomic RNA.”
Andrew H. (University of British Columbia)
“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
Caroline S. (Cornell University)
“It’s [an] excellent kit to work with tissues with high poly-phenols and acids.”
Arun D. (University of Missouri)
“The Zymo-Spin IC column is convenient.”
Lihua D. (National Institutes of Health)
Click here to submit your review of the RNA Clean & Concentrator™-5.