Quick-RNA™ MiniPrep (Plus)

Purify high-quality total RNA (including small/micro RNAs) from any cells, tissues, blood and biological fluids.
DNA-freeRNA is ready for use in any downstream application. DNase I included.

Product Size Catalog # Price Qty
NEW Quick-RNA™ MiniPrep Plus 50 Preps R1057
$262.00
NEW Quick-RNA™ MiniPrep Plus 200 Preps R1058
$835.00
Quick-RNA™ MiniPrep 50 Preps R1054
$209.00
Quick-RNA™ MiniPrep 200 Preps R1055
$675.00

About Quick-RNA™ MiniPrep (Plus)

MiniPrep Plus MiniPrep
Cells
Any tissue
(soft, lipid, fibrous, FFPE)
Whole blood
Cells
Soft tissue
Included
DNase I
gDNA removal column
Proteinase K
DNA/RNA Shield (for sample storage)
DNAse I
gDNA removal column

The Quick-RNA MiniPrep kits are innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg).  The procedure combines a unique buffer system with Zymo-Spin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes.

The procedure is simple.  Add the provided RNA Lysis Buffer to a sample, and then purify the RNA using the Zymo-Spin Columns.  The result is highly-concentrated, DNA-free RNA that is suitable for RT-PCR, hybridization, sequencing etc.  In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.


Sample Sources Any cells (animal, blood cells etc.), all tissues (tough-to-lyse, FFPE, etc.), blood, biological fluids, and enzymatic reactions (e.g., DNase I), samples in DNA/RNA Shield™, TRIzol® or RNAlater™.
Storage Samples homogenized in RNA Lysis Buffer are stable and can be stored frozen prior to purification.
RNA Purity High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations.
RNA Recovery Up to 100 µg RNA can be eluted into ≥30 µl RNase-free water allowing for a highly concentrated sample.
Sample Size Up to 107 cells or 50 mg tissue.
RNA Storage RNA is eluted with RNase-free water and can be stored frozen.
Equipment Needed Microcentrifuge
Sample Preservation DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.

Universal

The Quick-RNA™ MiniPrep Plus kit is universal and accommodates any sample input including cells, tissue, blood, etc.

High Quality RNA

High quality RNA is consistently isolated with the Quick-RNA™ MiniPrep Plus kit (left), not with kits as recommended by supplier Q (right). Quality assessed by Agilent 2200 TapeStation; red = low quality

DNA-free RNA

RNA isolated with the Quick-RNA™ MiniPrep Plus kit is DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106 human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments.

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“It is easy and fast and at the end you do have results. No DNA was detected.”
Ximena O. (Leibniz Institute for Zoo and Wildlife Research)
“Very quick protocol, only one tube to deal with”
Melissa P. (University of Texas)
“Protocol is more simple and much quicker than previous kits we were using.”
John C. (College of Dentistry, University of Florida)
Click here to submit your review of the Quick-RNA™ MiniPrep (Plus).