About ZR Soil/Fecal RNA MicroPrep™
The ZR Soil/Fecal RNA MicroPrep™ is an innovative product designed for the simple, reliable, and rapid isolation of total RNA including small RNAs (>17 nt) from various soil, sludge, sediment and/or fecal samples. The procedure successfully isolates RNA from tough-to-lyse bacteria, fungi, protozoa (protist), algae, etc. in soil, and host RNA from fecal samples.
Samples are added to the ZR BashingBead™ Lysis Tube with an optimally designed S/F RNA Lysis Buffer where microbes are then lysed by bead beating to extract total RNA. The Zymo-Spin™ column technology allows for quick filtration, genomic DNA removal from sample lysates, and isolation of the RNA. Zymo-Spin™ IV-HRC Spin Filter separates RT-PCR inhibitors (e.g., humic acids, polyphenols, tannins) and the total RNA is concentrated using the Zymo-Spin™ IC Column with a minimum elution volume of ≥6 µl.
The result is highly-concentrated, purified RNA that is a suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
|Format||Bead beating, spin column.|
|Equipment||Microcentrifuge, vortex, cell disrupter/pulverizer (optional).|
|Sample Sources||Bacteria, fungi, protozoa, and algae in soil, sludge or sediments, and bacteria, protist and/or host RNA from feces (mammalian, avian, etc.).|
|RNA Purity||High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations.|
|RNA Recovery||Up to 10 µg RNA can be eluted into ≥6 µl RNase-free water allowing for a highly concentrated sample.|
|Sample Size||≤250 mg|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70 ºC. The addition of RNase inhibitors is highly recommended for prolonged storage.|
Total RNA isolation of Arthrobacter sp. from 250 mg sludge using the ZR Soil/Fecal RNA MicroPrep™ in duplicate. ZR 1 kb DNA ladder, Zymo Research, Cat. No. M5006-50.
PCR amplification of Arthrobacter sp. rRNA transcript (361 bp fragment shown) in duplicate: ZR 100 bp DNA ladder, Zymo Research, Cat. No. M5005-50. PCR controls: Negative control - Total RNA isolation from Arthrobacter sp. in 250 mg sludge in duplicate (above). Positive control - Arthrobacter sp. genomic DNA. Negative control - Water.
|Click Here to download the protocol|