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DNA/RNA Shield™ Blood Collection Tubes

Nucleic Acid Stabilization at Ambient Temperature

  • • An evacuated blood tube filled with our novel DNA/RNA Shield™ collection and storage reagent
  • • Preserves nucleic acid at ambient temperatures for up to 30 days
  • • DNA and RNA can be isolated directly without precipitation or reagent removal
  • • Inactivates infectious agents (virus)

Microbial Inactivation

Viruses, bacteria and yeast are effectively inactivated by DNA/RNA Shield™. Samples containing the infectious agent (virus, bacteria, yeast) were treated with DNA/RNA Shield™ or mock (PBS) treated for 5 minutes. Titer (PFU) was subsequently determined by plaque assay. Validated by: Influenza A - D. Poole and Prof. A. Mehle, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison; Ebola (Kikwit) - L. Avena and Dr. A. Griffiths, Department of Virology and Immunology, Texas Biomedical Research Institute; HSV-1/2 - H. Oh, F. Diaz and Prof. D. Knipe, Virology Program, Harvard Medical School; E. coli, L. fermentum, B. subtilis, S. cerevisiae - Zymo Research Corporation)

*Disclaimer: This graph only displays results from E. coli inactivation. Each microbe was tested independently and were combined into one graph for brevity. Bacterial cultures were grown between 108 - 109 cells and yeast cultures were grown between 107 - 108 cells.

Streamlined Purification

Samples in DNA/RNA Shield™ can be input directly into most off-the-shelf purification kits, without the removal of the reagent, including:
Quick-DNA™ Universal (D4068), Quick-RNA™ (R1057)
ZR-Duet DNA/RNA™ (D7003), ZymoBIOMICS™ DNA Mini KIt (D4300)

High quality DNA and RNA is efffectively purified from blood stored in DNA/RNA Shield™ using the Quick-gDNA™ and Quick-RNA™ kits from Zymo Research respectively. High molecular weight DNA was intact with no apparent degradation. Also, RNA was high quality, DNA-free and incuded small RNAs when purified from DNA/RNA Shield™. This was not the case using another supplier's product (data not shown).

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