The Only Comprehensive Solution for Microbiomics and Metagenomics
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ZymoBIOMICS™ Microbial Community Standards
Find your bias &
Nucleic Acid Stabilization at Ambient Temperature
DNA and RNA in saliva and stool is effectively stabilized in DNA/RNA Shield™ at ambient temperature. Cellular RNA from human whole blood and spike-in DNA and RNA controls from saliva, stool and tissue purified at the indicated time points and analyzed by (RT)qPCR. Controls: HSV-1 and HIV (AcroMetrix, Life Technologies).
DNA/RNA Shield™ Preserves Microbial
Composition at Ambient Temperature
Microbial composition of stool is unchanged after one month at ambient temperature with DNA/RNA Shield™. Stool samples suspended in DNA/RNA Shield™ and stored at room temperature were compared to stool without preservative for one month. They were sampled at the indicated time points and processed with ZymoBIOMICS™ DNA Mini Kit. The extracted DNA was then subjected to microbial composition profiling via 16S rRNA gene targeted sequencing. Graphs show both phylum composition (left) and genus composition (right). Samples stored with DNA/RNA Shield™ had a constant microbial composition while the samples stored without shifted dramatically.
Viruses, bacteria and yeast are effectively inactivated by DNA/RNA Shield™. Samples containing the infectious agent (virus, bacteria, yeast) were treated with DNA/RNA Shield™ or mock (PBS) treated for 5 minutes. Titer (PFU) was subsequently determined by plaque assay. Validated by: Influenza A - D. Poole and Prof. A. Mehle, Department of Medical Microbiology and Immunology, University of Wisconsin, Madison; Ebola (Kikwit) - L. Avena and Dr. A. Griffiths, Department of Virology and Immunology, Texas Biomedical Research Institute; HSV-1/2 - H. Oh, F. Diaz and Prof. D. Knipe, Virology Program, Harvard Medical School; E. coli, L. fermentum, B. subtilis, S. cerevisiae - Zymo Research Corporation)
*Disclaimer: This graph only displays results from E. coli inactivation. Each microbe was tested independently and were combined into one graph for brevity. Bacterial cultures were grown between 108 - 109 cells and yeast cultures were grown between 107 - 108 cells.
ZymoBIOMICS™ DNA Mini Kit
ZymoBIOMICS™ DNA Mini Kit
- • Ultra-pure, inhibitor-free DNA from any sample (feces, soil, water, biofilms, swabs, body fluid, etc.) that is ideal for all downstream applications including PCR, arrays, 16s rRNA Gene Sequencing, and Shotgun Sequencing.
- • Innovative lysis system enables efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungus, protozoans, and algae for accurate microbial community profiling.
Accurate Community Profiling
Innovation. Pure & Simple.™
ZymoBIOMICS™ PCR PreMix and
Femto™ DNA Quantification Kits
- • Use the low-bioburden, ZymoBIOMICS™ PCR Pre-Mix to ensure non-biased quantification and microbiomic analysis
- • The Femto™ DNA Quantification Kits can reliably detect and quantify bacterial, fungal, and human DNA with high sensitivity and specificity to as little as 20 fg of DNA in 1 µl of eluate
Low-Bioburden DNA Polymerase
Accurately Quantify Bacterial, Fungal, or Human DNA
Validated, Non-Biased Workflows from Collection to Analysis
ZymoBIOMICS™ Services are validated using the ZymoBIOMICS™ Microbial Community Standards for unbiased, accurate community profiling
Comprehensive, Customizable Bioinformatics & Data Analysis
Our qualified service professionals are happy to help accelerate your research with services custom-tailored to meet your needs.
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