Maximizing the RNA-Seq library and data quality via quality control of input total RNA.
RNA-sequencing (RNA-Seq) has become increasingly popular for transcriptome profiling. The integrity and purity of the input RNA plays a critical role in the preparation of high-quality RNA-Seq libraries. Therefore, it is a good practice to perform quality control on the input RNA before proceeding to library preparation. Here, we provide some top recommendations to help preserve and verify the quality of input RNA for more robust RNA-Seq libraries.
- RNA extraction recommendations
- Suggestions for total RNA quality control
- Example data included for easier interpretation