Considerations for Maximizing the Quality of RNA-Seq Libraries and Sequencing Data

RNA-sequencing (RNA-seq) has become an increasingly popular technique for transcriptome profiling. The preparation of high-quality RNA-seq libraries is critical to the integrity and reliability of the sequencing data. Here we discuss some tips and tricks to help maximize the quality of RNA-seq libraries when preparing using a kit such as the Zymo-Seq RiboFree Total RNA Library Kit to ensure as high quality sequencing data as possible.

Application Note Highlights:

• RNA integrity and QC recommendations
• Tips for bead cleanup steps
• Suggestions on Library QC and Characterization