ZR Plasmid Miniprep™ - Classic

For purification of high quality, endotoxin-free plasmid DNA for restriction endonuclease digestion, DNA sequencing, transformation, cloning, transfection, in vitro transcription reactions, etc.

Innovative colored P1, P2, and P3 buffers for rapid identification of complete bacterial cell lysis and neutralization steps.

Unique column design: zero buffer retention and low (30 µl) elution volume.

Product Name	Size	Catalog #	Price
ZR Plasmid Miniprep™ - Classic	100 Preps.	D4015	$100.00
ZR Plasmid Miniprep™ - Classic	400 Preps.	D4016	$336.00
ZR Plasmid Miniprep™ - Classic	800 Preps.	D4054	$612.00

About ZR Plasmid Miniprep™ - Classic

The ZR Plasmid Miniprep™-Classic is designed for efficient isolation of plasmid DNA from E. Coli using a traditional 3-buffer (P1, P2, P3) procedure that is simple, rapid, user-friendly, and reliable. It features a modified alkaline lysis protocol together with a unique Fast-Spin column to yield high quality plasmid DNA in minutes. The buffers are color-coded (red, green, yellow) for easy determination of complete cell lysis and neutralization. The innovative Zymo-Spin™ llN columns yield endotoxin-free plasmid DNA. Plasmid DNA purified using the ZR Plasmid Miniprep™-Classic is well suited for use in restriction endonuclease digestion, sequencing, DNA ligation, cloning, PCR, bacterial transformation, transfection, etc.

Specifications

Format	Spin Column
DNA Recovery	≥ 30 µl
Processing Time	15 min
DNA Purity	Plasmid DNA which is well suited for ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. Typical A_(260/280) ≥ 1.8.
Plasmid DNA Size	Up to 25 kb.
Plasmid DNA Yield	Up to 25 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized.
Procedure	Performed at room temperature (15-30°C) using a centrifuge and/or a vacuum manifold.



Plasmid products. Restriction Endonuclease digestion of three different plasmids prepared using the ZR Plasmid Miniprep™-Classic, performed in triplicate. M: ZR 1 kb DNA marker (Zymo Research)	Sequence-quality DNA preparations. DNA sequencing chromatogram of plasmid DNA prepared using the ZR Plasmid Miniprep™-Classic.