About ZR-96 DNA Clean-up Kit™ (Shallow well format)
The ZR-96 DNA Clean-up Kit™ provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A™ Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.
|DNA Recovery||Typically, up to 5 µg total DNA (per well) can be eluted into as little as 30 - 40 µl water per sample. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
|Equipment||Centrifuge (with microplate carriers)|
|DNA Size Limits||75 bp-23 kb|
|Sample Sources||DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc.|
|DNA Purity||Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions.|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
High-throughput DNA processing. Crude preparations of a 3 kb plasmid DNA from bacterial lysates were processed using the ZR-96 DNA Clean-up Kit™. Following elution from the plate, 48 samples were then separated in a 0.8% (w/v) agarose gel.
|Kit / Catalog #||ZR-96 DNA Clean-up
Kit (D4017 and D4018)
|ZR-96 DNA Clean &
(D4023 and D4024)
|Binding Plate||Silicon-A™ Plate||Zymo-Spin™ I-96 Plate|
|Height of Binding Plate||19 mm (0.75 inches)||35 mm (1.38 inches)|
|Binding Plate/Collection Plate Assembly||43 mm (1.69 inches)||60 mm (2.36 inches)|
|Binding Cap./Minimum Elution Volume||5 µg/30 µl||5 µg/15 µl|
In a 1.5 ml microcentrifuge tube, add 2 volumes of DNA Binding Buffer to each volume of DNA sample. (e.g., 300 µl binding buffer to 150 µl sample). Mix briefly by vortexing.
Transfer sample mixtures to the wells of a Silicon-A™ Plate mounted onto a Collection Plate.
Centrifuge at ≥ 3,000 x g (5,000 x g max.) for 5 minutes until sample mixtures have been completely filtered. Discard the flow-through.
Add 300 µl Wash Buffer to each well of the Silicon-A™ Plate. Centrifuge at ≥ 3,000 x g for 5 minutes. Repeat wash step.
Add 30-40 µl water directly to the column matrix in each well. Transfer the Silicon-A™ Plate onto an Elution Plate and centrifuge at ≥ 3,000 x g for 3 minutes to elute the DNA.
Ultra-pure DNA in water is now ready for use.