5-hmC Glucosyltransferase

Specific modification of 5-hydroxymethylcytosine with glucose moiety

Product Size Catalog # Price Qty
5-hmC Glucosyltransferase (100 U) 100 units E2026
$125.00
5-hmC Glucosyltransferase (200 U) 200 units E2027
$207.00

About 5-hmC Glucosyltransferase

5-hmC Glucosyltransferase from Zymo Research is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety yielding glucosyl-5-hydroxymethylcytosine (Figure 1).

 

Figure 1: 5-hmC Glucosyltransferase transfers a glucose moeity from uridine diphosphoglucose (UDPG) onto preexisting 5-hydroxymethylcytosines within DNA.
 

Glucosylation of 5-hydroxymethylcytosine by 5-hmC Glucosyltransferase can be used for sequence specific and locus specific (see Cat. Nos. D5410 & D5411), as well as global quantification of 5-hydroxymethylcytosine.


Applications • Used in method for sequence and locus specfic detection of 5-hydroxymethylcytosine within DNA
• Global quantification of 5-hydroxymethylcytosine
Storage 5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Long term storage at -80°C is recommended. Avoid multiple freeze thawing.
Unit Definition One unit (1 U) is defined as the amount of enzyme needed to “protect” 1µg of 5hmC DNA Standard [D5405-3] from GlaI digestion via glucosylation in a reaction incubate at 30°C for 1 hour.
Standard Reaction Setup
Reagent Volume
DNA [100ng/µl] 10 µl
10x 5hmC GT Reaction Buffer 5 µl
10xUDPG [1mM] 5 µl
5hmC GT Enzyme 2 µl
ddH2O 28 µl
Total Volume 50 µl
Enzyme Concentration 2 units/µl

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