About 5-hmC Glucosyltransferase
5-hmC Glucosyltransferase from Zymo Research is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety yielding glucosyl-5-hydroxymethylcytosine (Figure 1).
Figure 1: 5-hmC Glucosyltransferase transfers a glucose moeity from uridine diphosphoglucose (UDPG) onto preexisting 5-hydroxymethylcytosines within DNA.
Glucosylation of 5-hydroxymethylcytosine by 5-hmC Glucosyltransferase can be used for sequence specific and locus specific (see Cat. Nos. D5410 & D5411), as well as global quantification of 5-hydroxymethylcytosine.
|Applications||• Used in method for sequence and locus specfic detection of 5-hydroxymethylcytosine within DNA • Global quantification of 5-hydroxymethylcytosine|
|Storage||5-hmC Glucosyltransferase is guaranteed for 12 months at -20°C. Long term storage at -80°C is recommended. Avoid multiple freeze thawing.|
|Unit Definition||One unit (1 U) is defined as the amount of enzyme needed to “protect” 1µg of 5hmC DNA Standard [D5405-3] from GlaI digestion via glucosylation in a reaction incubate at 30°C for 1 hour.|
|Standard Reaction Setup||
|Enzyme Concentration||2 units/µl|