About Atlantis dsDNase
Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes.
|Unit Definition||One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 mg/ml high MW DNA in 50 mM Na-acetate pH 5.0 and 5 mM MgCI2 (Kunitz, 1950).|
|Inactivation||5X MN Stop Buffer or EDTA|
|Specificity||Typical buffer consists of 20 mM Tris-HCI (pH 7.5) and 5 mM MgCI2.|
|Enzyme Concentration||0.1 U/µl|
|Optimum Reaction Temperature||42°-C|
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