Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS
Convenient 1 hour, single-enzyme digest vs. conventional 6-16 hours multi-step enzyme digestion protocols

Product Size Catalog # Price Qty
DNA Degradase Plus(250 U) 250 Units E2020
$136.00
DNA Degradase Plus(1000 U) 1,000 Units E2021
$435.00

About DNA Degradase Plus™

DNA Degradase Plus™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus™ is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.


Format Provided in solution (5 units/µl) w/ 10X Reaction Buffer
Storage Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be ≤ -70°C.
Unit Definition One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C.
Reaction Conditions DNA Degradase Plus™ in 1X DNA Degradase™ Reaction Buffer. Incubate reaction mixtures at 37°C for ≥1 hour.
Inactivation Heat-inactivate at 70°C for 20 minutes.
Standard Reaction Setup The setup (below) is an example of a typical DNA Degradase Plus™ reaction in a 25 µl final volume.
2 µl        DNA at 500 ng/µl
2.5 µl     10X DNA Degradase™ Reaction Buffer
1 µl        DNA Degradase Plus™ (5 units/µl)
19.5 µl   ddH2O
25 µl      Total Volume
  
Incubate at 37°C for 2 hours.
 

 

DNA Degradase Plus™ efficiently degrades DNA: 1 or 5 µg of λ DNA incubated with 5 U of DNA Degradase Plus™ in 25 µl reaction volume at 37°C for 1 hour. "M" is a 1 kb DNA ladder (Zymo Research).
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Featured Citations

The authors used DNA Degradase™ Plus to determine total methylation in genomic DNA from developing mouse embryonic tissue by mass spectrometry.
The authors sought to quantify levels of 5-mC in a number of yeast species using DNA Degradase™ Plus and LC-MS/MS.
DNA Degradase™ Plus was used to quantify the levels of 5-hmC and 5-mC in genomic DNA of tumor cells or cultured cell lines using a sensitive liquid chromatography-tandem quadrupole mass spectrometric method.
Authors sought to quantify levels of 5-mC in human placenta samples using LC-ESI/MS/MS and DNA Degradase™ Plus.
Genomic DNA from E. coli expressing DNA methyltransferases (MTases) from various bacterial strains was digested with DNA Degradase Plus™ to obtain nucleoside samples that can be analyzed by LC-MS. Analysis showed the presence of N6-methyl-2’-deoxyadenosine, which indicates the MTases are capable of N6-methyladenine modifications.

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