About Quick-gDNA™ Blood MidiPrep
Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to Quick-gDNA™ MidiPrep (D3100) for the replacement product.The Quick-gDNA™ Blood MidiPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.
|DNA Recovery||Up to 125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.|
|Processing Time||20 min|
|DNA Size Limits||Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.|
|Sample Sources||Up to 3 ml (see protocol) whole blood, plasma, or serum from humans, mice, rats, etc.|
|DNA Purity||High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8|
|Product Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.|
|Equipment Needed||Centrifuge or vacuum source and manifold, microcentrifuge, vortex|
Disconnect the Zymo-Spin™ V-E Column/Zymo-Midi Filter™ assembly and transfer the Zymo-Spin™ V-E Column to a Collection Tube. Spin at 10,000 x g for 1 minute in a microcentrifuge4 to remove residue from the column.
Add 300 µl DNA Pre-Wash Buffer to the column and spin at 10,000 x g for 1 minute. Discard the flow through.
Add 400 µl of g-DNA Wash Buffer to the column and centrifuge at 10,000 x g for one minute. Discard flow through and repeat wash step.
Transfer the Zymo-Spin™ V-E Column to a 1.5 ml microcentrifuge tube and add 150 µl DNA Elution Buffer directly to the column matrix5 and allow column to stand for 1 minute at room temperature. Centrifuge at 10,000 x g for 1 minute to elute the DNA6. The eluted DNA can be used immediately for molecular based applications or stored ≤-20oC for future use.