Quick-gDNA™ Blood MidiPrep

Quick method for the purification of high quality DNA from up to 3 ml whole blood, plasma, and serum, in less than 20 minutes using innovative Fast-Spin column technology. Up to 125 µg/prep.
Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).
Unique extraction technology excludes the use of Proteinase K and organic denaturants.
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

Product Size Catalog # Price Qty
Quick-gDNA™ Blood MidiPrep 25 Preps. D3074
$106.00

About Quick-gDNA™ Blood MidiPrep

Note: This product will be discontinued within the next 3 months (November 30th, 2016) or while supplies last. Please refer to Quick-gDNA™ MidiPrep (D3100) for the replacement product.

The Quick-gDNA™ Blood MidiPrep is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, and plasma.


Format Spin Column
DNA Recovery Up to 125 µg total DNA is eluted into ≥150 µl DNA Elution Buffer or water. Human whole blood will typically yield 3-7 µg DNA per 100 µl blood sampled.
Processing Time 20 min
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Up to 3 ml (see protocol) whole blood, plasma, or serum from humans, mice, rats, etc.
DNA Purity High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280<1.8
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS.
Equipment Needed Centrifuge or vacuum source and manifold, microcentrifuge, vortex

Step 1

Add 12 ml of Genomic Lysis Buffer to 3 ml1 of blood, serum, or plasma (4:1).  Mix completely by vortexing 4-6 seconds, then let stand 5-10 minutes at room temperature.

 Note:  Add 12 ml Genomic Lysis Buffer to all samples < 3 ml.    

Step 2

Transfer the mixture to a Zymo-Spin™ V-E Column/Zymo-Midi Filter assembly into a 50 ml tube2.  Centrifuge at ≥1,000 x g (2,000 x g max.) for 5 minutes3.

Note:  If using a vacuum manifold, the processing capacity is reduced to 2 ml of blood, serum, or plasma + 12 ml Genomic Lysis Buffer per prep.  This filtration step may take up to twenty minutes when using vacuum.

Step 3

Disconnect the Zymo-Spin™ V-E Column/Zymo-Midi Filter™ assembly and transfer the Zymo-Spin™ V-E Column to a Collection Tube.  Spin at 10,000 x g for 1 minute in a microcentrifuge4 to remove residue from the column.

Step 4

Add 300 µl DNA Pre-Wash Buffer to the column and spin at 10,000 x g for 1 minute.  Discard the flow through.

Step 5

Add 400 µl of g-DNA Wash Buffer to the column and centrifuge at 10,000 x g for one minute.  Discard flow through and repeat wash step.

Step 6

Transfer the Zymo-Spin™ V-E Column to a 1.5 ml microcentrifuge tube and add 150 µl DNA Elution Buffer directly to the column matrix5 and allow column to stand for 1 minute at room temperature.  Centrifuge at 10,000 x g for 1 minute to elute the DNA6.  The eluted DNA can be used immediately for molecular based applications or stored ≤-20oC for future use.

For specific notes and additional information, please see the product protocol PDF.
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