About Quick-DNA™ 96 Plus Kit
Note: The Quick-DNA™ Universal 96 Kit (D4070/D4071) has been renamed to Quick-DNA™ 96 Plus Kit; all components and protocols are the same and have not been modified.
The Quick-DNA™ 96 Plus Kit is the easiest method for high-throughput total DNA extraction (e.g., genomic, mitochondrial, viral) from any biological fluid, cell culture, or solid tissue sample. Innovative reagents and Zymo-SpinTM technologies allow for ultra-pure and concentrated genomic DNA ˃ 50 kb to be eluted in as little as 15 µl. Zymo-Spin™ Plates ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
|Equipment||Water bath or heat block (55oC), microcentrifuge, and vortex.|
|Sample Sources||See full protocol for details|
|DNA Purity||High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230 ≥ 2.0).|
|Elution Volume||DNA can be eluted into as little as 15 µl DNA Elution Buffer or water.|
|DNA Yield||The DNA binding capacity of each well is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.|
|DNA Size||Capable of recovering genomic and mitochondrial DNA sized fragments ˃ 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.|
|DNA Applications||DNA isolated using the Quick-DNA™ 96 Plus Kit can be used for life-science research, genotyping, livestock breeding, veterinary research, and routine applied testing among a variety of other applications.|
|DNA Types||The Quick-DNA™ 96 Plus Kit will isolate total DNA including genomic, mitochondrial, plasmid, viral, parasitic, etc. Not recommended for small cell-free DNA isolation from urine, serum, and plasma.|
|Workflow Overview||Utilize a Proteinase K Digestion and Zymo-Spin™ Column for effective recovery of DNA. See page 5 for more information.|
High Quality DNA Obtained from a Wide Range of Biological Samples Using the Quick-DNA™ 96 Plus Kit. DNA purified using the Quick-DNA™ 96 Plus Kit is ultrapure, highly concentrated, and ready for all downstream applications. Input DNA was standardized to 300 ng and analyzed in a 1% (w/v) TAE/agarose/EtBr gel (shown above). The size marker “M” is a 1 kb ladder (Zymo Research).
HSV-1 Viral DNA is Effectively Isolated from Plasma Using the Quick-DNA™ 96 Plus Kit. A dilution series of HSV-1 spiked into porcine plasma and extracted using the Quick-DNA™ 96 Plus Kit shows effective purification and subsequent qPCR amplification, even at a 20,000:1 dilution. The no template controls did not amplify even after 50 cycles.
Resuspend ≤ 1 x 106 mammalian or insect cell pellets using 50 µl DNA Elution Buffer or an isotonic buffer (e.g. PBS).
Overnight Proteinase K digestions at 55ºC are possible without affecting the integrity of the DNA