ZR Fecal DNA MicroPrep™

Rapid methods for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes including those from humans, birds, rats, mice, cattle, etc.
Ultra-high density BashingBeads™ are fracture resistant and chemically inert.
Fast-Spin column and unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Product Size Catalog # Price Qty
ZR Fecal DNA MicroPrep™ 50 Preps. D6012

About ZR Fecal DNA MicroPrep™

The ZR Fecal DNA MicroPrep™ is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Format Spin Column
DNA Recovery Up to 5 μg total DNA is eluted into ≥ 10 μl DNA Elution Buffer per sample.
Processing Time 15 min
Equipment Microcentrifuge, vortex, cell disruptor/pulverizer (optional)
DNA Size Limits Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
Sample Sources Bacterial, protist, as well as host DNA can be effectively isolated from a ≤150 mg sample of mammalian feces.
DNA Purity High quality, inhibitor-free DNA is eluted with DNA Elution Buffer suitable for the amplification of bacterial, protist, and/or mammalian templates. A260/A280 >1.8

Comparison of DNA yields from rat feces using the ZR Fecal DNA MiniPrep™ and kits from suppliers A and B. Equivalent amounts of feces were processed using each kit and then equal volumes of eluted DNA were analyzed in a 0.8% (w/v) agarose/ethidium bromide gel. Samples were processed in triplicate.

Step 1

Add up to 150 mg of fecal sample to a ZR BashingBead™ Lysis Tube.  Add 750 µl Lysis Solution to the tube.

Step 2

Secure in a bead beater fitted with a 2 ml tube holder assembly (e.g., Disruptor Genie™) and process at maximum speed for 5 minutes.

Processing times may be as little as 40 seconds when using high-speed cell disrupters (e.g., the portable Xpedition™ Sample Processor, page 6, FastPrepâ-24, or similar).  See manufacturer’s literature for operating information.


Step 3

Centrifuge the ZR BashingBead™ Lysis Tube in a microcentrifuge at ≥10,000 x g for 1 minute.

Step 4

Transfer up to 400 µl supernatant to a Zymo-Spin™ IV Spin Filter (orange top) in a Collection Tube and centrifuge at 7,000 rpm (~7,000 x g) for 1 minute.

Step 5

Add 1,200 µl of Fecal DNA Binding Buffer to the filtrate in the Collection Tube from Step 4.

Step 6

Transfer 800 µl of the mixture from Step 5 to a Zymo-Spin™ IC Column in a Collection Tube and centrifuge at 10,000 x g for 1 minute.  

Step 7

Discard the flow through from the Collection Tube and repeat Step 6.

Step 8

Add 200 µl DNA Pre-Wash Buffer to the Zymo-Spin™ IC Column in a new zCollection Tube and centrifuge at 10,000 x g for 1 minute.  

Step 9

Add 500 µl Fecal DNA Wash Buffer to the Zymo-Spin™ IC Column and centrifuge at 10,000 x g for 1 minute.

Step 10

Transfer the Zymo-Spin™ IC Column to a clean 1.5 ml microcentrifuge tube and add 20 µl (10 µl minimum) DNA Elution Buffer directly to the column matrix.  Centrifuge at 10,000 x g for 30 seconds to elute the DNA.

Step 11

Transfer the eluted DNA from Step 10 to a prepared Zymo-Spin™ IV-µHRC Spin Filter (yellow top) (see above) in a clean 1.5 ml microcentrifuge tube and centrifuge at exactly 8,000 x g for 1 minute.  The filtered DNA is now suitable for PCR and other downstream applications.     

For specific notes and additional information, please see the product protocol PDF.
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