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ZymoPURE-EndoZero™ Plasmid Maxiprep Kit

The fastest spin-column based procedure for purifying up to 1.2 mg of endotoxin-free plasmid DNA.
Routinely achieve endotoxin levels of ≤ 0.025 EU/µg of plasmid DNA in 20 minutes.
Plasmid DNA that is ideal for transfection in sensitive cells and in vivo research.

Product Size Catalog # Price Qty
ZymoPURE EndoZero™ Plasmid Maxiprep 10 Preps D4205

About ZymoPURE-EndoZero™ Plasmid Maxiprep Kit

The ZymoPURE EndoZero Plasmid Maxiprep kit features a spin column-based method for the purification of up to 1.2 mg of high-quality endotoxin-free plasmid DNA in less than 20 minutes. The isolated plasmid DNA is ready for immediate use, and does not require subsequent precipitation steps.

ZymoPURE technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl).  In addition, the kit incorporates our unique EndoZero spin-columns to reduce endotoxin levels down to 0.025 EU/µg of plasmid DNA without lengthy incubations, gravity flow anion-exchange columns, expensive chromatography columns, or time-consuming centrifugation steps.  The result is plasmid DNA suitable for transfection, restriction endonuclease digestion, in vivo studies, bacterial transformation, PCR amplification, DNA sequencing, and other sensitive downstream applications.

As an added convenience, the ZymoPURE-EndoZero Plasmid Maxiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization.

Processing Time 20 min
Equipment Microcentrifuge and vacuum/vacuum manifold (recommended) or swinging bucket centrifuge.
DNA Purity Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection, transformation, sequencing, restriction endonuclease digestion, in vitro transcription, in vivo studies, and other sensitive applications. Typical Abs260/280 ≥ 1.8 and Abs260/230 ≥ 2.0. Endotoxin levels ≤ 0.025 EU/µg of plasmid DNA.
Plasmid DNA Size Up to 25 kb
Plasmid DNA Yield Up to 1.2 mg per preparation (Actual yield is dependent on the plasmid copy number, culture growth conditions, and strain of E. coli utilized)
Recovery Volume ≥ 200 µl of ZymoPURE™ Elution Buffer or DNase free water

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