About Quick-RNA™ MicroPrep
The Quick-RNA™ MicroPrep is an innovative product designed for the easy, reliable, and rapid isolation of total RNA from cultured cells or solid tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Fast-Spin column technology to yield high quality RNA in minutes. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest and then purify the RNA using the provided spin column. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
Specifications
| Format | Spin column |
|---|---|
| Processing Time | 10 min |
| Sample Sources | Cells from culture or tissue samples, buccal cells, buffy coat, plasma, serum, and other biological liquids. Not compatible with whole blood. (For purification of RNA from whole blood, see the ZR Whole-Blood RNA MiniPrep (R1020)) |
| RNA Purity | High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
| RNA Recovery | Up to 5 μg RNA can be eluted into ≥ 6 μl RNase-free water allowing for a highly concentrated sample. |
| Sample Size | 1 to 105 cells (≤100 μl plasma, serum). |
| RNA Storage | -70ºC |
| Equipment Needed | Microcentrifuge |
|
Human RNA isolated from cell culture with the Quick-RNA™ MiniPrep Kit. M: 1 kb DNA marker (Zymo Research); 1, 2: duplicate RNA preparations.
|
PCR amplification of a ß-actin transcript (353 bp fragment shown) following RNA isolation from human epithelial cells (HCT 116) with the Quick-RNA™ MidiPrep: RT-PCR (lanes 1, 2), PCR negative control (RNA template; lanes 3, 4). M1 and M2 are 100 bp and 1 kb DNA Markers, respectively (Zymo Research).
|
Your shopping cart is empty.
